Cited 68 times in
Anti-inflammatory Effect of Capsaicin in Helicobacter pylori-Infected Gastric Epithelial Cells
DC Field | Value | Language |
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dc.contributor.author | 김지현 | - |
dc.contributor.author | 이용찬 | - |
dc.date.accessioned | 2014-12-21T16:38:47Z | - |
dc.date.available | 2014-12-21T16:38:47Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1083-4389 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96081 | - |
dc.description.abstract | BACKGROUND AND AIM: Capsaicin, the main pungent ingredient of hot red and chilli pepper, has been considered as not only a cytoprotective but also a detrimental agent to the gastric mucosa. However, the effect and mechanism of capsaicin that modulate the induction of pro-inflammatory cytokine in Helicobacter pylori-infected epithelial cells have not been investigated previously. Herein, we demonstrated that capsaicin inhibited the release of pro-inflammatory cytokine, interleukin-8 (IL-8) by H. pylori-infected gastric epithelial cells through nuclear factor-kappaB (NF-kappaB) signal pathway. MATERIALS AND METHODS: AGS or MKN45 cells as gastric epithelial cells and Vac A+, CagA+ wild-type H. pylori strain ATCC 49503 were used. Gastric epithelial cells were pre-treated with various concentrations of capsaicin and infected with H. pylori for different periods of time to determine IL-8 concentrations in culture supernatant by an ELISA assay. We measured IL-8 mRNA transcripts in H. pylori-infected gastric epithelial cells co-treated with capsaicin by reverse transcriptase-polymerase chain reaction analysis. We performed electrophoretic mobility shift assay to examine the NF-kappaB DNA binding activity with capsaicin and immunofluorescence microscopy to examine nuclear staining of p65. We also performed immunoblotting for IkappaB, IKK activity with capsaicin. RESULTS: Capsaicin inhibits H. pylori-induced IL-8 production by gastric epithelial cells in dose- and time-dependent manner. Capsaicin as low as 100 micromol/L significantly inhibited IL-8 production in H. pylori-infected MKN45 cells (43.2% of control) at 24 hours incubation, whereas inhibited IL-8 production in H. pylori-infected AGS cells (70% of control). We confirmed that capsaicin inhibited IL-8 mRNA expression after infection of gastric epithelial cells with H. pylori for 6 hours. The addition of capsaicin (100 micromol/L) suppressed H. pylori-induced NF-kappaB activation in gastric epithelial cells at 1 hour post-infection. We also found that the degradation of IkappaB and IKK activation were inhibited by capsaicin. CONCLUSIONS: Nontoxic dose of capsaicin inhibited H. pylori-induced IL-8 production by gastric epithelial cells through the modulation of IkappaB-, NF-kappaB-, and IL-8 pathways. We conclude that capsaicin can be proposed as a potential anti-inflammatory drug by inhibition of the production of IL-8 in H. pylori-infected gastric epithelium. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 510~517 | - |
dc.relation.isPartOf | HELICOBACTER | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anti-Inflammatory Agents, Non-Steroidal/pharmacology* | - |
dc.subject.MESH | Capsaicin/pharmacology* | - |
dc.subject.MESH | Epithelial Cells/drug effects | - |
dc.subject.MESH | Epithelial Cells/immunology* | - |
dc.subject.MESH | Epithelial Cells/microbiology | - |
dc.subject.MESH | Gastric Mucosa/cytology | - |
dc.subject.MESH | Gastric Mucosa/drug effects | - |
dc.subject.MESH | Gastric Mucosa/immunology* | - |
dc.subject.MESH | Gastric Mucosa/microbiology | - |
dc.subject.MESH | Helicobacter Infections/microbiology | - |
dc.subject.MESH | Helicobacter pylori/drug effects | - |
dc.subject.MESH | Helicobacter pylori/pathogenicity* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-8/antagonists & inhibitors* | - |
dc.subject.MESH | Interleukin-8/biosynthesis | - |
dc.subject.MESH | NF-kappa B/metabolism | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.title | Anti-inflammatory Effect of Capsaicin in Helicobacter pylori-Infected Gastric Epithelial Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | In Ohk Lee | - |
dc.contributor.googleauthor | Kwang Hyoung Lee | - |
dc.contributor.googleauthor | Yong Chan Lee | - |
dc.contributor.googleauthor | Yeun Jung Choi | - |
dc.contributor.googleauthor | Jie Hyun Kim | - |
dc.contributor.googleauthor | Jae Hee Pyo | - |
dc.identifier.doi | 10.1111/j.1523-5378.2007.00521.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02988 | - |
dc.contributor.localId | A00996 | - |
dc.relation.journalcode | J00981 | - |
dc.identifier.eissn | 1523-5378 | - |
dc.identifier.pmid | 17760719 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1523-5378.2007.00521.x/abstract | - |
dc.contributor.alternativeName | Kim, Ji Hyun | - |
dc.contributor.alternativeName | Lee, Yong Chan | - |
dc.contributor.affiliatedAuthor | Lee, Yong Chan | - |
dc.contributor.affiliatedAuthor | Kim, Ji Hyun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 12 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 510 | - |
dc.citation.endPage | 517 | - |
dc.identifier.bibliographicCitation | HELICOBACTER, Vol.12(5) : 510-517, 2007 | - |
dc.identifier.rimsid | 35419 | - |
dc.type.rims | ART | - |
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