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Potent HAT Inhibitory Effect of Aqueous Extract from Bellflower(platycodon grandiflorum) Roots on Androgen Receptor-mediated Transcriptional Regulation

Authors
 Yoo-Hyun Lee  ;  Yong Jun Kim  ;  Ho-Geun Yoon  ;  Hong Yon Cho  ;  Ha-Il Kim 
Citation
 FOOD SCIENCE AND BIOTECHNOLOGY, Vol.16(3) : 457-462, 2007 
Journal Title
 FOOD SCIENCE AND BIOTECHNOLOGY 
ISSN
 1226-7708 
Issue Date
2007
MeSH
bellflower root ; histone acetyltransferase ; androgen receptor
Abstract
Histone acetyltransferase (HAT) is a family of enzymes that regulate histone acetylation. Dysfunction of HAT plays a critical role in the development of cancer. Here we have screened the various plant extracts to find out the potent HAT inhibitors. The bellflower(platycodon grandiflorum) root have exhibited approximately 30% of the inhibitory effects on HAT activity, especially p300 and CBP (CREB-binding protein) at the concentration of 100 μg/mL. The cell viability was decreased approximately 52% in LNCaP cell for 48 hr incubation. Furthermore, mRNA level of 3 androgen receptor target genes, PSA, NKX3.1, and TSC22 were decreased with bellflower root extract treatment (100 μg/mL) in the presence of androgen. In ChIP assay, the acetylation of histone H3 and H4 in PSA promoter region was dramatically repressed by bellflower root treatment, but not TR target gene, D1. Therefore, the potent HAT inhibitory effect of bellflower root led to the decreased transcription of AR target genes and prostate cancer cell growth with the repression of histone hyperacetylation.
Full Text
http://www.dbpia.co.kr/Article/1606665
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ha Il(김하일)
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
Lee, Yoo Hyun(이유현)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96036
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