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Interleukin-1β suppresses epithelial sodium channel β-subunit expression and ENaC-dependent fluid absorption in human middle ear epithelial cells

Authors
 Jae Young Choi  ;  Yoon-Seok Choi  ;  Joo-Heon Yoon  ;  Hyun Seung Choi  ;  Eun Jin Son  ;  Su Jin Kim 
Citation
 EUROPEAN JOURNAL OF PHARMACOLOGY, Vol.567(1-2) : 19-25, 2007 
Journal Title
EUROPEAN JOURNAL OF PHARMACOLOGY
ISSN
 0014-2999 
Issue Date
2007
MeSH
Absorption ; Bicarbonates ; Buffers ; Cell Line ; Ear, Middle/cytology ; Ear, Middle/metabolism* ; Epithelial Cells/cytology ; Epithelial Cells/metabolism* ; Epithelial Sodium Channels/biosynthesis* ; Humans ; Immunohistochemistry ; Interleukin-1beta/pharmacology ; Interleukin-1beta/physiology* ; Protein Isoforms/biosynthesis ; Signal Transduction ; Sodium Channel Blockers/pharmacology ; Solutions ; Water/metabolism*
Abstract
Recent reports have shown that cytokines inhibit fluid absorption by suppressing Na+ channel activity in various epithelia. In this study, we investigated the role of epithelial sodium channel (ENaC) in fluid absorption in normal human middle ear epithelial (NHMEE) cells, as well as the effects of Interleukin (IL)-1β on ENaC expression and fluid absorption in NHMEE cells. We confirmed that ENaC α, β and γ were predominantly expressed on the apical surface of the NHMEE cells by immunocytochemistry. Addition of amiloride, a potent ENaC blocker, to apical membranes of NHMEE cells decreased the fluid absorption rate in a dose-dependent manner. Treatment with 10 ng/ml IL-1β for 24 h suppressed ENaC β expression, the ENaC-dependent short-circuit current (Isc), and ENaC-dependent fluid absorption. When the NHMEE cells were pretreated with a phospholipase C (PLC)inhibitor (U73122, 10 μM), a protein kinase C (PKC) inhibitor (Calphostin C, 0.1 μM), or extracellular signal regulated kinase (ERK) 1/2 inhibitor (PD98059, 10 μM), the amiloride-sensitive currents in IL-1β-treated cells were reversed to control levels; an effect not seen with SB202190 (an inhibitor of p38 mitogen-activated protein (MAP) kinase) or SP600125 (a reversible inhibitor of c-Jun N-terminal kinase). In this study we showed that ENaC is essential for fluid absorption in NHMEE cells and that IL-1β suppresses the ENaC-dependent current via the PLC-PKC-ERK1/2 pathway. These results suggest that IL-1β may contribute to fluid retention in otitis media with effusion by changing electrolyte transport and reducing middle ear epithelial fluid absorption.
Full Text
http://www.sciencedirect.com/science/article/pii/S0014299907004669
DOI
10.1016/j.ejphar.2007.04.026
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Joo Heon(윤주헌)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96008
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