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Vasorelaxation by Samhwangsasim-tang, an herb medicine, is associated with decreased phosphorylation of the myosin phosphatase target subunit

Authors
 Fanxue Jin  ;  Heung-Mook Shin  ;  In Kyeom Kim  ;  Young-Ho Lee  ;  Young Mi Seok  ;  Jee In Kim  ;  En-yue Yang  ;  Inji Baek  ;  Su Bun Jeon 
Citation
 ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, Vol.24(3) : 199-205, 2007 
Journal Title
 ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 
ISSN
 1382-6689 
Issue Date
2007
Abstract
Samhwangsasim-tang (SST) is a widely used herbal medicine with vasodilatory actions in oriental countries. We hypothesized that SST modulates vascular contractility by decreasing phosphorylation of the myosin phosphatase target subunit. Rat aortic ring preparations were mounted in organ baths and subjected to contractions or relaxations. Phosphorylation of 20kDa myosin light chains (MLC(20)) and MYPT1, a target subunit of myosin phosphate 1, were examined with immunoblots. SST relaxed aortic ring preparations precontracted with phenylephrine whether endothelium was intact or denuded. Treatment of aortic rings with N(ω)-nitro-l-arginine methyl ester (l-NAME), an inhibitor of endothelial nitric oxide synthase or methylene blue, an inhibitor of guanylyl cyclase, did not affect the relaxing action of SST. Furthermore, SST inhibited vascular contractions induced by NaF or phenylephrine, but not by phorbol dibutyrate. SST also decreased vascular tension precontracted by 8.0mmol/L NaF or 1.0μmol/L phenylephrine, but not by 1.0μmol/L phorbol dibutyrate. In vascular strips, SST decreased the phosphorylation level of both MLC(20) and MYPT1 induced by 8.0mmol/L NaF. In conclusion, SST inhibited vascular contraction by decreasing phosphorylation of the myosin phosphatase target subunit.
Full Text
http://www.sciencedirect.com/science/article/pii/S1382668907000749
DOI
10.1016/j.etap.2007.05.004
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Lee, Young Ho(이영호) ORCID logo https://orcid.org/0000-0002-5749-1045
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95970
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