Cited 22 times in
Prevalence and mechanisms of decreased susceptibility to carbapenems in Klebsiella pneumoniae isolates
DC Field | Value | Language |
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dc.contributor.author | 이경원 | - |
dc.date.accessioned | 2014-12-21T16:34:42Z | - |
dc.date.available | 2014-12-21T16:34:42Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0732-8893 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95950 | - |
dc.description.abstract | In this study, we examined the prevalence of and mechanisms of decreased susceptibility to either imipenem or meropenem in Klebsiella pneumoniae isolates. A total of 230 clinical isolates of K. pneumoniae were collected from 13 clinical laboratories from a nationwide distribution. The MICs of imipenem and meropenem were determined by the agar dilution method. To characterize the isolates with decreased susceptibility to carbapenems (MICs of >2 μg/mL), we performed polymerase chain reaction amplification of a variety of β-lactamase genes, isoelectric focusing, and outer membrane profile analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry. Three isolates (BD6, BD8, and KN16) exhibited decreased susceptibility to carbapenems with imipenem MICs of 1, 4, and 8 μg/mL and meropenem MICs of 4, 8, and 4, respectively. Isolate BD6 produced blaTEM-1, blaSHV-12, and blaOXA-17; isolate BD8 produced blaGES-3, blaSHV-12, and blaOXA-17; and isolate KN16 produced blaTEM-11, blaSHV-12, and blaDHA-1. In all the 3 isolates, OmpK35 porin was not expressed, and in 1 isolate (KN16), OmpK36 was not expressed either. The prevalence of decreased susceptibility to carbapenems was low (1.3%), and none of them showed overt resistance to carbapenems. Decreased susceptibility to carbapenems can occur in K. pneumoniae when blaGES-3, blaTEM-11, blaSHV-12, blaOXA-17, and/or blaDHA-1 are produced in combination with porin loss. In addition, to our knowledge, this is the 1st report of blaOXA-17 in Enterobacteriaceae. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 85~91 | - |
dc.relation.isPartOf | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Anti-Bacterial Agents/pharmacology* | - |
dc.subject.MESH | Drug Resistance, Bacterial* | - |
dc.subject.MESH | Electrophoresis, Polyacrylamide Gel | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Imipenem/pharmacology* | - |
dc.subject.MESH | Isoelectric Focusing | - |
dc.subject.MESH | Klebsiella Infections/epidemiology* | - |
dc.subject.MESH | Klebsiella Infections/microbiology | - |
dc.subject.MESH | Klebsiella pneumoniae/drug effects* | - |
dc.subject.MESH | Klebsiella pneumoniae/isolation & purification | - |
dc.subject.MESH | Korea/epidemiology | - |
dc.subject.MESH | Mass Spectrometry | - |
dc.subject.MESH | Meropenem | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Porins/metabolism | - |
dc.subject.MESH | Prevalence | - |
dc.subject.MESH | Sequence Analysis, DNA | - |
dc.subject.MESH | Thienamycins/pharmacology* | - |
dc.subject.MESH | beta-Lactamases/genetics | - |
dc.title | Prevalence and mechanisms of decreased susceptibility to carbapenems in Klebsiella pneumoniae isolates | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Soo-Young Kim | - |
dc.contributor.googleauthor | Yeon-Joon Park | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.contributor.googleauthor | Jin-Young Yoo | - |
dc.contributor.googleauthor | Jong-Bok Yoon | - |
dc.contributor.googleauthor | Yong Sung Park | - |
dc.contributor.googleauthor | Han Sik Kim | - |
dc.contributor.googleauthor | Jin-Kyung Yu | - |
dc.identifier.doi | 10.1016/j.diagmicrobio.2006.05.008 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02649 | - |
dc.relation.journalcode | J00731 | - |
dc.identifier.eissn | 1879-0070 | - |
dc.identifier.pmid | 16854555 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0732889306002008 | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 57 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 85 | - |
dc.citation.endPage | 91 | - |
dc.identifier.bibliographicCitation | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.57(1) : 85-91, 2007 | - |
dc.identifier.rimsid | 35325 | - |
dc.type.rims | ART | - |
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