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Sphingosine-1-phosphate initiates rapid retraction of pseudopodia by localized RhoA activation

Authors
 Eunjin Koh  ;  Timothy Clair  ;  Mary L. Stracke  ;  David D. Roberts  ;  Elliott Schiffmann  ;  Russell W. Bandle  ;  Raejean Hermansen 
Citation
 CELLULAR SIGNALLING, Vol.19(6) : 1328-1338, 2007 
Journal Title
 CELLULAR SIGNALLING 
ISSN
 0898-6568 
Issue Date
2007
Abstract
Lysophosphatidic acid (LPA) stimulates sphingosine-1-phosphate (S1P)-sensitive motility in NIH3T3 clone7 cells. S1P inhibits motility only when added to the bottom well of the Boyden chamber, suggesting that pseudopodia can respond to their microenvironment. In order to study and localize this effect, we utilized a Transwell insert system to isolate pseudopodia. LPA stimulates protrusion of pseudopodia that are enriched in RhoA compared to cell bodies. Removal of LPA results in slow retraction with loss of vinculin-rich adhesion complexes and prolonged activation of RhoA. However, RhoA, ROCK and mDia are not required for this process. In contrast, rapid retraction, induced by adding S1P to the bottom well, is associated with a quick spike of activated RhoA and coalescence of adhesion complexes that colocalize with the ends of stress fibers. S1P-induced retraction requires RhoA and ROCK but is only delayed by inhibition of mDia. These data indicate that pseudopodia sense and integrate signals initiated by localized bioactive lipids, affecting both cellular polarity and their own function in motility.
Full Text
http://www.sciencedirect.com/science/article/pii/S0898656807000290
DOI
10.1016/j.cellsig.2007.01.005
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Koh, Eun Jin(고은진) ORCID logo https://orcid.org/0000-0001-8967-6266
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95823
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