Cited 9 times in
New Fast BiFC Plasmid Assay System for in Vivo Protein-Protein Interactions
DC Field | Value | Language |
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dc.contributor.author | 허만욱 | - |
dc.contributor.author | 김명화 | - |
dc.contributor.author | 노희은 | - |
dc.date.accessioned | 2014-12-21T16:30:24Z | - |
dc.date.available | 2014-12-21T16:30:24Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1015-8987 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95821 | - |
dc.description.abstract | In this age of massive genetic and protein information, a fast and reliable method of studying in vivo protein-protein interactions is necessary. We have developed a novel system that can overcome limitations of existing assay methods. This new method adopts two existing systems for fast analysis of diverse protein-protein interactions. For rapid, large-scale cloning, we adopted the Gateway system and developed novel destination vectors containing YFP N-terminus (YN) or YFP C-terminus (YC) to visualize protein-protein interactions in vivo using bimolecular fluorescence complementation (BiFC). Using this system, we investigated molecular interactions among the three POZ-domain regulatory proteins mAPM-1, LRF, KLHL10 that belong to a subgroup of human POZ-domain proteins, and showed that the POZ-domains of mAPM-1, LRF and KLHL10 could form both homodimers and heterodimers. This new method is a highly efficient, sensitive and specific assay method for protein-protein interaction in vivo. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 703~714 | - |
dc.relation.isPartOf | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | New Fast BiFC Plasmid Assay System for in Vivo Protein-Protein Interactions | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Myung-Hwa Kim | - |
dc.contributor.googleauthor | Hee-Eun Roh | - |
dc.contributor.googleauthor | Man-Wook Hur | - |
dc.contributor.googleauthor | Min-Nyung Lee | - |
dc.identifier.doi | 10.1159/000110431 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04350 | - |
dc.contributor.localId | A00430 | - |
dc.contributor.localId | A01306 | - |
dc.relation.journalcode | J00501 | - |
dc.identifier.eissn | 1421-9778 | - |
dc.identifier.url | http://www.karger.com/Article/Abstract/110431 | - |
dc.contributor.alternativeName | Hur, Man Wook | - |
dc.contributor.alternativeName | Kim, Myung Hwa | - |
dc.contributor.alternativeName | Roh, Hee Eun | - |
dc.contributor.affiliatedAuthor | Hur, Man Wook | - |
dc.contributor.affiliatedAuthor | Kim, Myung Hwa | - |
dc.contributor.affiliatedAuthor | Roh, Hee Eun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 20 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 703 | - |
dc.citation.endPage | 714 | - |
dc.identifier.bibliographicCitation | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, Vol.20(6) : 703-714, 2007 | - |
dc.identifier.rimsid | 53227 | - |
dc.type.rims | ART | - |
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