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Anti-Histone Acetyltransferase Activity from Allspice Extracts Inhibits Androgen Receptor-Dependent Prostate Cancer CellGrowth

Authors
 Yoo-Hyun LEE  ;  Soon Won HONG  ;  Ho-Geun YOON  ;  Chang-Hoon KIM  ;  Ha-Il KIM  ;  Jiemin WONG  ;  Myung Gu JUNG  ;  Han-Cheon KIM  ;  Hong Yon CHO  ;  Woojin JUN 
Citation
 BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Vol.71(11) : 2712-2719, 2007 
Journal Title
 BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 
ISSN
 0916-8451 
Issue Date
2007
Abstract
Histone acetylation depends on the activity of two enzyme families, histone acetyltransferase (HAT) and deacetylase (HDAC). In this study, we screened various plant extracts to find potent HAT inhibitors. Hot water extracts of allspice inhibited HAT activity, especially p300 and CBP (40% at 100 μg/ml). The mRNA levels of two androgen receptor (AR) regulated genes, PSA and TSC22, decreased with allspice treatment (100 μg/ml). Importantly, in IP western analysis, AR acetylation was dramatically decreased by allspice treatment. Furthermore, chromatin immunoprecipitation indicated that the acetylation of histone H3 in the PSA and B2M promoter regions was also repressed. Finally, allspice treatment reduced the growth of human prostate cancer cells, LNCaP (50% growth inhibition at 200 μg/ml). Taken together, our data indicate that the potent HAT inhibitory activity of allspice reduced AR and histone acetylation and led to decreased transcription of AR target genes, resulting in inhibition of prostate cancer cell growth.
Files in This Item:
T200701062.pdf Download
DOI
10.1271/bbb.70306
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ha Il(김하일)
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
Lee, Yoo Hyun(이유현)
Hong, Soon Won(홍순원) ORCID logo https://orcid.org/0000-0002-0324-2414
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95722
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