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Protective effect of heat shock protein 27 using protein transduction domain-mediated delivery on ischemia/reperfusion heart injury

DC Field Value Language
dc.contributor.author강석민-
dc.contributor.author김진배-
dc.contributor.author장양수-
dc.contributor.author정남식-
dc.date.accessioned2014-12-21T16:26:28Z-
dc.date.available2014-12-21T16:26:28Z-
dc.date.issued2007-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/95698-
dc.description.abstractHeat shock protein 27 (HSP27) is an intracellular stress protein with the cytoprotective effect for a variety of noxious stresses. In this study, using a protein delivery system, we demonstrated the potential cytoprotective effect of HSP27 as a therapeutic protein in cardiac cells and ischemia/reperfusion animal model. We constructed a recombinant HSP27 fused to the protein transduction domain (PTD) derived from HIV-1 TAT protein. Purified recombinant TAT-HSP27 protein was efficiently delivered to H9c2 cells, and its transduction showed cytoprotective effect against the hypoxic stress. Moreover, transduction of TAT-HSP27 also attenuated hypoxia-induced apoptosis, which was accompanied by reduced caspase-3 activity. In addition, intraperitoneal injection of TAT-HSP27 into rat resulted in efficient protein transduction in heart tissues, decreased infarcted myocardium (control vs TAT-HSP27, 39.1% vs 29.5%, P<0.05) and preserved heart function (fractional shortening, 15.6% vs 33.4%, P<0.05), as determined at 7 d after I/R. These results suggest that the PTD-mediated delivery of HSP27 protein may represent a potential therapeutic strategy as protein drug for ischemic heart diseases.-
dc.description.statementOfResponsibilityopen-
dc.format.extent399~404-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleProtective effect of heat shock protein 27 using protein transduction domain-mediated delivery on ischemia/reperfusion heart injury-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorJun Hye Kwon-
dc.contributor.googleauthorJin-Bae Kim-
dc.contributor.googleauthorJi Hyung Chung-
dc.contributor.googleauthorYangsoo Jang-
dc.contributor.googleauthorNamsik Chung-
dc.contributor.googleauthorSeok-Min Kang-
dc.contributor.googleauthorKyung-Hye Lee-
dc.identifier.doi10.1016/j.bbrc.2007.09.001-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00037-
dc.contributor.localIdA01016-
dc.contributor.localIdA03448-
dc.contributor.localIdA03585-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X07019304-
dc.contributor.alternativeNameKang, Seok Min-
dc.contributor.alternativeNameKim, Jin Bae-
dc.contributor.alternativeNameJang, Yang Soo-
dc.contributor.alternativeNameChung, Nam Sik-
dc.contributor.affiliatedAuthorKang, Seok Min-
dc.contributor.affiliatedAuthorKim, Jin Bae-
dc.contributor.affiliatedAuthorJang, Yang Soo-
dc.contributor.affiliatedAuthorChung, Nam Sik-
dc.rights.accessRightsnot free-
dc.citation.volume363-
dc.citation.number2-
dc.citation.startPage399-
dc.citation.endPage404-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.363(2) : 399-404, 2007-
dc.identifier.rimsid45032-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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