Cited 40 times in
Protective effect of heat shock protein 27 using protein transduction domain-mediated delivery on ischemia/reperfusion heart injury
DC Field | Value | Language |
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dc.contributor.author | 강석민 | - |
dc.contributor.author | 김진배 | - |
dc.contributor.author | 장양수 | - |
dc.contributor.author | 정남식 | - |
dc.date.accessioned | 2014-12-21T16:26:28Z | - |
dc.date.available | 2014-12-21T16:26:28Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95698 | - |
dc.description.abstract | Heat shock protein 27 (HSP27) is an intracellular stress protein with the cytoprotective effect for a variety of noxious stresses. In this study, using a protein delivery system, we demonstrated the potential cytoprotective effect of HSP27 as a therapeutic protein in cardiac cells and ischemia/reperfusion animal model. We constructed a recombinant HSP27 fused to the protein transduction domain (PTD) derived from HIV-1 TAT protein. Purified recombinant TAT-HSP27 protein was efficiently delivered to H9c2 cells, and its transduction showed cytoprotective effect against the hypoxic stress. Moreover, transduction of TAT-HSP27 also attenuated hypoxia-induced apoptosis, which was accompanied by reduced caspase-3 activity. In addition, intraperitoneal injection of TAT-HSP27 into rat resulted in efficient protein transduction in heart tissues, decreased infarcted myocardium (control vs TAT-HSP27, 39.1% vs 29.5%, P<0.05) and preserved heart function (fractional shortening, 15.6% vs 33.4%, P<0.05), as determined at 7 d after I/R. These results suggest that the PTD-mediated delivery of HSP27 protein may represent a potential therapeutic strategy as protein drug for ischemic heart diseases. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 399~404 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Protective effect of heat shock protein 27 using protein transduction domain-mediated delivery on ischemia/reperfusion heart injury | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Jun Hye Kwon | - |
dc.contributor.googleauthor | Jin-Bae Kim | - |
dc.contributor.googleauthor | Ji Hyung Chung | - |
dc.contributor.googleauthor | Yangsoo Jang | - |
dc.contributor.googleauthor | Namsik Chung | - |
dc.contributor.googleauthor | Seok-Min Kang | - |
dc.contributor.googleauthor | Kyung-Hye Lee | - |
dc.identifier.doi | 10.1016/j.bbrc.2007.09.001 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00037 | - |
dc.contributor.localId | A01016 | - |
dc.contributor.localId | A03448 | - |
dc.contributor.localId | A03585 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X07019304 | - |
dc.contributor.alternativeName | Kang, Seok Min | - |
dc.contributor.alternativeName | Kim, Jin Bae | - |
dc.contributor.alternativeName | Jang, Yang Soo | - |
dc.contributor.alternativeName | Chung, Nam Sik | - |
dc.contributor.affiliatedAuthor | Kang, Seok Min | - |
dc.contributor.affiliatedAuthor | Kim, Jin Bae | - |
dc.contributor.affiliatedAuthor | Jang, Yang Soo | - |
dc.contributor.affiliatedAuthor | Chung, Nam Sik | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 363 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 399 | - |
dc.citation.endPage | 404 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.363(2) : 399-404, 2007 | - |
dc.identifier.rimsid | 45032 | - |
dc.type.rims | ART | - |
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