Cited 29 times in
Change of E-cadherin by hepatocyte growth factor and effects on the prognosis of hypopharyngeal carcinoma
DC Field | Value | Language |
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dc.contributor.author | 최은창 | - |
dc.date.accessioned | 2014-12-21T16:24:04Z | - |
dc.date.available | 2014-12-21T16:24:04Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1068-9265 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95622 | - |
dc.description.abstract | BACKGROUND: Hepatocyte growth factor (HGF) is known to induce scattering in various epithelial cells, and E-cadherin plays important roles in the maintenance of cell-cell adhesion. However, the mechanisms surrounding these actions are not fully understood. Therefore, we examined how HGF affects the expression and distribution of E-cadherin. In addition, we observed the relationship between prognosis and modulation of E-cadherin by HGF in hypopharyngeal carcinoma. METHODS: Tumor tissues from 66 patients with hypopharyngeal squamous cell carcinoma were evaluated for the expression of HGF, its receptor (c-Met), and E-cadherin. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot test were performed on hypopharyngeal cancer tissues. The association and changes of E-cadherin with HGF treatment in a hypopharyngeal cancer cell line were investigated by RT-PCR, Western blot analysis, inhibition assay, immunofluorescence staining, and invasion assay. RESULTS: E-cadherin expression was found in 87.9% of squamous cell carcinomas; these could be further classified as membranous type (46.9%) or nonmembranous type (53.1%). The expression of HGF in tumors with nonmembranous type E-cadherin expression was far higher than in tumors with membranous expression. Nonmembranous type E-cadherin expression correlated significantly with lymph node metastasis, distant metastasis, and recurrence (P < .05). HGF decreased the expression of E-cadherin and induced the translocation of E-cadherin to the cytoplasm. HGF and E-cadherin neutralizing antibody stimulated dispersion, and HGF significantly enhanced the invasion of hypopharyngeal cancer cells in a dose-dependent manner (P < .05). CONCLUSIONS: These results suggest that HGF can modulate the expression and intracellular localization of E-cadherin in hypopharyngeal cancer cells. In addition, these results indicate that changes in E-cadherin by HGF can affect the prognosis of hypopharyngeal carcinoma. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1565~1574 | - |
dc.relation.isPartOf | ANNALS OF SURGICAL ONCOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Change of E-cadherin by hepatocyte growth factor and effects on the prognosis of hypopharyngeal carcinoma | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | Chul-Ho Kim | - |
dc.contributor.googleauthor | JangHee Kim | - |
dc.contributor.googleauthor | Eun Chang Choi | - |
dc.contributor.googleauthor | Hison Kahng | - |
dc.identifier.doi | 10.1245/s10434-006-9320-5 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04161 | - |
dc.relation.journalcode | J00179 | - |
dc.identifier.eissn | 1534-4681 | - |
dc.identifier.url | http://link.springer.com/article/10.1245%2Fs10434-006-9320-5 | - |
dc.contributor.alternativeName | Choi, Eun Chang | - |
dc.contributor.affiliatedAuthor | Choi, Eun Chang | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 14 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1565 | - |
dc.citation.endPage | 1574 | - |
dc.identifier.bibliographicCitation | ANNALS OF SURGICAL ONCOLOGY, Vol.14(5) : 1565-1574, 2007 | - |
dc.identifier.rimsid | 44973 | - |
dc.type.rims | ART | - |
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