Cited 167 times in
Integrated Portable Polymerase Chain Reaction-Capillary Electrophoresis Microsystem for Rapid Forensic Short Tandem Repeat Typing
DC Field | Value | Language |
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dc.contributor.author | 신경진 | - |
dc.date.accessioned | 2014-12-21T16:22:58Z | - |
dc.date.available | 2014-12-21T16:22:58Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95587 | - |
dc.description.abstract | A portable forensic genetic analysis system consisting of a microfluidic device for amplification and separation of short tandem repeat (STR) fragments as well as an instrument for chip operation and four-color fluorescence detection has been developed. The microdevice performs polymerase chain reaction (PCR) in a 160-nL chamber and capillary electrophoresis (CE) in a 7-cm-long separation channel. The instrumental design integrates PCR thermal cycling, electrophoretic separation, pneumatic valve fluidic control, and four-color laser excited fluorescence detection. A quadruplex Y-chromosome STR typing system consisting of amelogenin and three Y STR loci (DYS390, DYS393, DYS439) was developed and used for validation studies. The multiplex amplification of these 4 loci with 35 PCR cycles followed by CE separation and 4-color fluorescence detection was completed in 1.5 h. All the amplicons can be detected with a limit of detection of 20 copies of male standard DNA in the reactor. Real-world forensic analyses of oral swab and human bone extracts from case evidence were also successfully performed. Mixture analysis demonstrated that a balanced profile can be obtained even at a male-to-female template ratio of 1:10. The successful development and operation of this portable PCR−CE system establishes the feasibility of rapid point-of-analysis DNA typing of forensic casework, of mass disaster samples or of individuals at a security checkpoint. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1881~1889 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Integrated Portable Polymerase Chain Reaction-Capillary Electrophoresis Microsystem for Rapid Forensic Short Tandem Repeat Typing | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Forensic Medicine (법의학) | - |
dc.contributor.googleauthor | Peng Liu | - |
dc.contributor.googleauthor | Tae Seok Seo | - |
dc.contributor.googleauthor | Richard A. Mathies | - |
dc.contributor.googleauthor | James R. Scherer | - |
dc.contributor.googleauthor | Kyoung-Jin Shin | - |
dc.contributor.googleauthor | Nathaniel Beyor | - |
dc.identifier.doi | 10.1021/ac061961k | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02085 | - |
dc.relation.journalcode | J00136 | - |
dc.identifier.eissn | 1520-6882 | - |
dc.identifier.url | http://pubs.acs.org/doi/abs/10.1021/ac061961k | - |
dc.contributor.alternativeName | Shin, Kyoung Jin | - |
dc.contributor.affiliatedAuthor | Shin, Kyoung Jin | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 79 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1881 | - |
dc.citation.endPage | 1889 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, Vol.79(5) : 1881-1889, 2007 | - |
dc.identifier.rimsid | 43351 | - |
dc.type.rims | ART | - |
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