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Expression and localization of surfactant proteins in human nasal epithelium

Authors
 Jin Kook Kim  ;  Sung-Shik Kim  ;  Joo-Heon Yoon  ;  Jeung-Gweon Lee  ;  Chang-Hoon Lee  ;  Jae Hoon Cho  ;  Chang-Hoon Kim  ;  Keung Won Rha 
Citation
 American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol.292(4) : 879-884, 2007 
Journal Title
 American Journal of Physiology - Lung Cellular and Molecular Physiology 
ISSN
 1040-0605 
Issue Date
2007
Abstract
Surfactant proteins (SPs), designated SP-A, SP-B, SP-C, and SP-D, play an important role in surfactant metabolism and host defense mechanisms in the lung. This study investigates expression of the different SP types in human nasal mucosa and cultured normal human nasal epithelial (NHNE) cells and whether the expression of SP mRNA is influenced by the degree of mucociliary differentiation. RT-PCR was performed with mRNA from cultured NHNE cells and nasal mucosa. Immunohistochemical staining for SPs was performed on nasal mucosa specimens. Western blot analysis was performed on cell lysates from cultured NHNE cells. SP-A2, SP-B, and SP-D mRNAs were expressed in normal NHNE cells and human nasal mucosa. SPs were localized in ciliated cells of the surface epithelium and serous acini of the submucosal glands. SP-A, SP-B, and SP-D proteins were expressed in cultured NHNE cells. The degree of mucociliary differentiation influenced expression of the SP gene. We demonstrate that SP-A, SP-B, and SP-D are expressed in human nasal mucosa and cultured NHNE cells. Further study of the functional role of SPs in the upper airway is required.
Files in This Item:
T200700533.pdf Download
DOI
10.1152/ajplung.00156.2006
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
Lee, Chang Hoon(이창훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95562
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