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Concerted action of sulfiredoxin and peroxiredoxin I protects against alcohol-induced oxidative injury in mouse liver

DC Field Value Language
dc.contributor.author길인섭-
dc.contributor.author배수한-
dc.contributor.author성수행-
dc.contributor.author이서구-
dc.date.accessioned2014-12-20T17:54:43Z-
dc.date.available2014-12-20T17:54:43Z-
dc.date.issued2011-
dc.identifier.issn0270-9139-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/95490-
dc.description.abstractPeroxiredoxins (Prxs) are peroxidases that catalyze the reduction of reactive oxygen species (ROS). The active site cysteine residue of members of the 2-Cys Prx subgroup (Prx I to IV) of Prxs is hyperoxidized to cysteine sulfinic acid (Cys-SO(2) ) during catalysis with concomitant loss of peroxidase activity. Reactivation of the hyperoxidized Prx is catalyzed by sulfiredoxin (Srx). Ethanol consumption induces the accumulation of cytochrome P450 2E1 (CYP2E1), a major contributor to ethanol-induced ROS production in the liver. We now show that chronic ethanol feeding markedly increased the expression of Srx in the liver of mice in a largely Nrf2-dependent manner. Among Prx I to IV, only Prx I was found to be hyperoxidized in the liver of ethanol-fed wildtype mice, and the level of Prx I-SO(2) increased to ≈30% to 50% of total Prx I in the liver of ethanol-fed Srx(-/-) mice. This result suggests that Prx I is the most active 2-Cys Prx in elimination of ROS from the liver of ethanol-fed mice and that, despite the up-regulation of Srx expression by ethanol, the capacity of Srx is not sufficient to counteract the hyperoxidation of Prx I that occurs during ROS reduction. A protease protection assay revealed that a large fraction of Prx I is located together with CYP2E1 at the cytosolic side of the endoplasmic reticulum membrane. The selective role of Prx I in ROS removal is thus likely attributable to the proximity of Prx I and CYP2E1. CONCLUSION: The pivotal functions of Srx and Prx I in protection of the liver in ethanol-fed mice was evident from the severe oxidative damage observed in mice lacking either Srx or Prx I.-
dc.description.statementOfResponsibilityopen-
dc.format.extent945~953-
dc.relation.isPartOfHEPATOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHChemical and Drug Induced Liver Injury/metabolism-
dc.subject.MESHChemical and Drug Induced Liver Injury/prevention & control*-
dc.subject.MESHCytochrome P-450 CYP2E1/metabolism-
dc.subject.MESHEthanol/toxicity*-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHOxidation-Reduction-
dc.subject.MESHOxidoreductases Acting on Sulfur Group Donors/deficiency-
dc.subject.MESHOxidoreductases Acting on Sulfur Group Donors/metabolism*-
dc.subject.MESHPeroxiredoxin III-
dc.subject.MESHPeroxiredoxins/metabolism*-
dc.subject.MESHReactive Oxygen Species/metabolism-
dc.titleConcerted action of sulfiredoxin and peroxiredoxin I protects against alcohol-induced oxidative injury in mouse liver-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorSoo Han Bae-
dc.contributor.googleauthorSu Haeng Sung-
dc.contributor.googleauthorEun Jung Cho-
dc.contributor.googleauthorSe Kyoung Lee-
dc.contributor.googleauthorHye Eun Lee-
dc.contributor.googleauthorHyun Ae Woo-
dc.contributor.googleauthorDae-Yeul Yu-
dc.contributor.googleauthorIn Sup Kil-
dc.contributor.googleauthorSue Goo Rhee-
dc.identifier.doi10.1002/hep.24104-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00281-
dc.contributor.localIdA01798-
dc.contributor.localIdA01948-
dc.contributor.localIdA02847-
dc.relation.journalcodeJ00985-
dc.identifier.eissn1527-3350-
dc.identifier.pmid21319188-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/hep.24104/abstract-
dc.contributor.alternativeNameKil, In Sup-
dc.contributor.alternativeNameBae, Soo Han-
dc.contributor.alternativeNameSung, Su Haeng-
dc.contributor.alternativeNameRhee, Sue Goo-
dc.contributor.affiliatedAuthorKil, In Sup-
dc.contributor.affiliatedAuthorBae, Soo Han-
dc.contributor.affiliatedAuthorSung, Su Haeng-
dc.contributor.affiliatedAuthorRhee, Sue Goo-
dc.rights.accessRightsnot free-
dc.citation.volume53-
dc.citation.number3-
dc.citation.startPage945-
dc.citation.endPage953-
dc.identifier.bibliographicCitationHEPATOLOGY, Vol.53(3) : 945-953, 2011-
dc.identifier.rimsid40849-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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