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CD8 T-cell activation in mice injected with a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean Plasmodium vivax.
DC Field | Value | Language |
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dc.contributor.author | 김태윤 | - |
dc.date.accessioned | 2014-12-20T17:53:42Z | - |
dc.date.available | 2014-12-20T17:53:42Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95457 | - |
dc.description.abstract | Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8(+) T-cells and CD4(+) T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8(+) cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, Protozoan/administration & dosage | - |
dc.subject.MESH | Antigens, Protozoan/genetics | - |
dc.subject.MESH | Antigens, Protozoan/immunology* | - |
dc.subject.MESH | CD8-Positive T-Lymphocytes/immunology* | - |
dc.subject.MESH | COS Cells | - |
dc.subject.MESH | Cercopithecus aethiops | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lymphocyte Activation | - |
dc.subject.MESH | Malaria, Vivax/immunology* | - |
dc.subject.MESH | Malaria, Vivax/parasitology | - |
dc.subject.MESH | Membrane Proteins/administration & dosage | - |
dc.subject.MESH | Membrane Proteins/genetics | - |
dc.subject.MESH | Membrane Proteins/immunology* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Plasmodium vivax/genetics | - |
dc.subject.MESH | Plasmodium vivax/immunology* | - |
dc.subject.MESH | Protozoan Proteins/administration & dosage | - |
dc.subject.MESH | Protozoan Proteins/genetics | - |
dc.subject.MESH | Protozoan Proteins/immunology* | - |
dc.subject.MESH | Protozoan Vaccines/administration & dosage | - |
dc.subject.MESH | Protozoan Vaccines/genetics | - |
dc.subject.MESH | Protozoan Vaccines/immunology* | - |
dc.subject.MESH | Vaccines, DNA/administration & dosage | - |
dc.subject.MESH | Vaccines, DNA/genetics | - |
dc.subject.MESH | Vaccines, DNA/immunology* | - |
dc.title | CD8 T-cell activation in mice injected with a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean Plasmodium vivax. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Hyo-Jin Kim | - |
dc.contributor.googleauthor | Bong-Kwang Jung | - |
dc.contributor.googleauthor | Jin-Joo Lee | - |
dc.contributor.googleauthor | Kyoung-Ho Pyo | - |
dc.contributor.googleauthor | Tae Yun Kim | - |
dc.contributor.googleauthor | Byung-il Choi | - |
dc.contributor.googleauthor | Tae Woo Kim | - |
dc.contributor.googleauthor | Hajime Hisaeda | - |
dc.contributor.googleauthor | Kunisuke Himeno | - |
dc.contributor.googleauthor | Eun-Hee Shin | - |
dc.contributor.googleauthor | Jong-Yil Chai | - |
dc.identifier.doi | 10.3347/kjp.2011.49.1.85. | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01077 | - |
dc.relation.journalcode | J01957 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.identifier.pmid | 21461275 | - |
dc.subject.keyword | CD8+ T-cell | - |
dc.subject.keyword | DNA vaccine | - |
dc.subject.keyword | Plasmodium vivax | - |
dc.subject.keyword | apical membrane antigen (AMA) | - |
dc.subject.keyword | gene gun | - |
dc.contributor.alternativeName | Kim, Tae Yun | - |
dc.contributor.affiliatedAuthor | Kim, Tae Yun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 49 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 85 | - |
dc.citation.endPage | 90 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, Vol.49(1) : 85-90, 2011 | - |
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