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Mass imaging of iron oxide nanoparticles inside cells for in vitro cytotoxicity

Authors
 Shon, Hyun Kyong  ;  Park, Jungsin  ;  Choi, Inhong  ;  Park, Hyun Min  ;  Moon, Dae Won  ;  Lee, Tae Geol 
Citation
 Journal of Nanoscience and Nanotechnology, Vol.11(1) : 638-641, 2011 
Journal Title
 Journal of Nanoscience and Nanotechnology 
ISSN
 1533-4880 
Issue Date
2011
Abstract
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging analysis was performed on murine macrophage cells treated with various concentrations of iron oxide (Fe3O4) nanoparticles, which are used as MRI contrast agents. First, murine macrophage cells were seeded on a slide glass for 24 hrs and treated with varying concentrations of Fe3O4 nanoparticles for 24 hrs. To expose a cross section of each cell and obtain a distribution of the nanoparticles inside the cells, the cells were sputtered using Bi ions after which the cross section of each cell was scanned and imaged using the focused cluster ion beam with a spatial resolution of 300 nm. Fe3O4 nanoparticles were found mainly in the cytoplasm region of the cells, not in the nucleus region of cells, suggesting that the uptake of the Fe3O4 nanoparticles were into the cytoplasm of cell, not into the nucleus of cell. Based on these observations, our protocol using mass imaging analysis would be a useful addition to the study of in vitro nanoparticle cytotoxicity
Full Text
http://www.ingentaconnect.com/content/asp/jnn/2011/00000011/00000001/art00114
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실)
Yonsei Authors
최인홍(Choi, In Hong) ORCID logo https://orcid.org/0000-0001-9851-0137
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95453
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