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Flt3 Signaling-Dependent Dendritic Cells Protect against Atherosclerosis

 Jae-Hoon Choi  ;  Cheolho Cheong  ;  Durga B. Dandamudi  ;  Chae Gyu Park  ;  Anthony Rodriguez  ;  Saurabh Mehandru  ;  Klara Velinzon  ;  In-Hyuk Jung  ;  Ji-Young Yoo  ;  Goo Taeg Oh  ;  Ralph M. Steinman 
 IMMUNITY, Vol.35(5) : 819-831, 2011 
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Animals ; Antigens, CD/metabolism ; Aorta/drug effects ; Aorta/immunology ; Atherosclerosis/genetics ; Atherosclerosis/immunology* ; Atherosclerosis/pathology ; Dendritic Cells/drug effects ; Dendritic Cells/immunology* ; Dendritic Cells/metabolism ; Gene Expression Regulation/immunology ; Leukocyte Reduction Procedures ; Macrophage Colony-Stimulating Factor/metabolism ; Macrophages/immunology ; Membrane Proteins/pharmacology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Knockout ; Monocytes/immunology ; Signal Transduction* ; fms-Like Tyrosine Kinase 3/genetics ; fms-Like Tyrosine Kinase 3/metabolism*
Early events in atherosclerosis occur in the aortic intima and involve monocytes that become macrophages. We looked for these cells in the steady state adult mouse aorta, and surprisingly, we found a dominance of dendritic cells (DCs) in the intima. In contrast to aortic adventitial macrophages, CD11c(+)MHC II(hi) DCs were poorly phagocytic but were immune stimulatory. DCs were of two types primarily: classical Flt3-Flt3L signaling-dependent, CD103(+)CD11b(-) DCs and macrophage-colony stimulating factor (M-CSF)-dependent, CD14(+)CD11b(+)DC-SIGN(+) monocyte-derived DCs. Both types expanded during atherosclerosis. By crossing Flt3(-/-) to Ldlr(-/-) atherosclerosis-prone mice, we developed a selective and marked deficiency of classical CD103(+) aortic DCs, and they were associated with exacerbated atherosclerosis without alterations in blood lipids. Concomitantly, the Flt3(-/-)Ldlr(-/-) mice had fewer Foxp3(+) Treg cells and increased inflammatory cytokine mRNAs in the aorta. Therefore, functional DCs are dominant in normal aortic intima and, in contrast to macrophages, CD103(+) classical DCs are associated with atherosclerosis protection.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
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