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Evaluation of a rapid assay for identification of Mycobacterium tuberculosis grown in solid and liquid media

Authors
 Ang, C.F.  ;  Cajucom, M.A.M.  ;  Kim, Y.  ;  Bang, H.  ;  Lee, H.  ;  Cho, S.N.  ;  Montalban, C.S. 
Citation
 International Journal of Tuberculosis and Lung Disease, Vol.15(11) : 1475-1477, 2011 
Journal Title
 International Journal of Tuberculosis and Lung Disease 
ISSN
 1027-3719 
Issue Date
2011
MeSH
Antigens, Bacterial/analysis* ; Bacterial Proteins/genetics ; Bacteriological Techniques* ; Culture Media* ; DNA-Directed RNA Polymerases ; Humans ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/growth & development ; Mycobacterium tuberculosis/immunology* ; Mycobacterium tuberculosis/isolation & purification ; Polymerase Chain Reaction ; Predictive Value of Tests ; Reagent Kits, Diagnostic* ; Sensitivity and Specificity ; Tuberculosis/diagnosis* ; Tuberculosis/microbiology
Keywords
IDENTIFICATION ; MPT64 ANTIGEN ; MYCOBACTERIUM TUBERCULOSIS
Abstract
An MPT64 antigen detection assay, the SD Bioline TB Ag MPT64 Rapid kit, was evaluated for the identification of Mycobacterium tuberculosis grown in both solid and liquid media. The rapid test showed positive results in 132 of 133 (99.2%) M. tuberculosis cultures grown on Ogawa medium and 136/143 (95.1%) cultures grown in liquid culture medium, resulting in an overall sensitivity of 97.1%. All 18 non-tuberculous mycobacteria were found to be negative by the rapid test, indicating a specificity of 100%. The rapid test seems useful for the initial confirmation of M. tuberculosis in acidfast bacilli positive cultures.
Full Text
http://dx.doi.org/10.5588/ijtld.10.0709
DOI
10.5588/ijtld.10.0709
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sang Nae(조상래)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95242
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