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Puromycin aminonucleoside 투여에 따른 사구체 족세포 β-catenin의 변화

Other Titles
 The Change of Podocyte beta-Catenin by Puromycin Aminonucleoside. 
Authors
 최지영 ; 안은미 ; 하태선 ; 신재일 ; 박혜영 
Citation
 Journal of the Korean Society of Pediatric Nephrology (대한소아신장학회지), Vol.15(2) : 138~145, 2011 
Journal Title
 Journal of the Korean Society of Pediatric Nephrology  (대한소아신장학회지) 
ISSN
 1226-5292 
Issue Date
2011
Abstract
PURPOSE: To test whether the expression of beta-catenin, a component of podocyte as a filtration molecule, would be altered by puromycin aminonucleoside (PAN) in the cultured podocyte in vitro. METHODS: We cultured rat glomerular epithelial cells (GEpC) with various concentrations of PAN and examined the distribution of beta-catenin by confocal microscope and measured the change of beta-catenin expression by Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: We found that beta-catenin relocalized from peripheral cytoplasm to inner cytoplasm, therefore, intercellular separations were seen in confluently cultured cells by high concentrations of PAN in immunofluorescence views. In Western blotting of GEpC, PAN (50 microg/mL) decreased beta-catenin expression by 34.9% at 24 hrs and 34.3% at 48 hrs, compared to those in without PAN condition (P<0.05). In RT-PCR, high concentrations (50 microg/mL) of PAN also decreased beta-catenin mRNA expression similar to protein suppression by 25.4% at 24 hrs and 51.8% at 48 hrs (P<0.05). CONCLUSION: Exposure of podocytes to PAN in vitro relocates beta-catenin internally and reduces beta-catenin mRNA and protein expression, which could explain the development of proteinuria in experimental PAN-induced nephropathy
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94940
DOI
10.3339/jkspn.2011.15.2.138
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pediatrics
Yonsei Authors
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