Cited 12 times in
Effects of the immobilization of heparin and rhPDGF-BB to titanium surfaces for the enhancement of osteoblastic functions and anti-inflammation.
DC Field | Value | Language |
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dc.contributor.author | 심준성 | - |
dc.date.accessioned | 2014-12-20T17:33:20Z | - |
dc.date.available | 2014-12-20T17:33:20Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 2005-7806 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94819 | - |
dc.description.abstract | PURPOSE: This study was to investigate the effects of recombinant human platelet-derived growth factor (rhPDGF-BB) and heparin to titanium surfaces for enhancement of osteoblastic functions and inhibition of inflammation activity. MATERIALS AND METHODS: The anodized titanium discs, not coated with any material, were used as a control group. In heparinized- Ti group, dopamine was anchored to the surface of Ti substrates, and coated with heparin. In PDGF-Ti group, rhPDGF-BB was immobilized onto heparinized Ti surface. The surface morphologies were investigated by the scanning electron microscope in each group. The release kinetics of rhPDGF-BB were analyzed, and cytotoxicity tests for each group were conducted. The biocompatibilities were characterized by measuring cell proliferation, alkaline phosphatase activity, and calcium deposition using MG-63 cells. Statistical comparisons were carried out by one-way ANOVA tests. Differences were considered statistically significant at (*)P<.05 and (**)P<.001. RESULTS: The combination of rhPDGF-BB and heparin stimulated alkaline phosphatase activity and OCN mRNA expression in osteoblastic cells ((*)P<.05 and (**)P<.001). MG-63 cells grown on PDGF-Ti had significantly higher amounts of calcium deposition than those grown on anodized Ti ((**)P<.001). Heparinized Ti was more anti-inflammatory compared to anodized Ti, when exposed to lipopolysaccharide using the transcript levels of TNF-α and IL-6 of proinflammatory cytokine ((*)P<.05 and (**)P<.001). CONCLUSION: The result of this study demonstrated that the incorporation of rhPDGF-BB and heparin onto Ti surface enhanced osteoblastic functions and inhibited inflammation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | JOURNAL OF ADVANCED PROSTHODONTICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Effects of the immobilization of heparin and rhPDGF-BB to titanium surfaces for the enhancement of osteoblastic functions and anti-inflammation. | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Prosthodontics (보철과학) | - |
dc.contributor.googleauthor | Jung-Bo Huh | - |
dc.contributor.googleauthor | Jeong-Yol Lee | - |
dc.contributor.googleauthor | Kyung-Lae Lee | - |
dc.contributor.googleauthor | Sung-Eun Kim | - |
dc.contributor.googleauthor | Mi-Jung Yun | - |
dc.contributor.googleauthor | Ji-Suk Shim | - |
dc.contributor.googleauthor | June-Sung Shim | - |
dc.contributor.googleauthor | Sang-Wan Shin | - |
dc.identifier.doi | 10.4047/jap.2011.3.3.152 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02211 | - |
dc.relation.journalcode | J01223 | - |
dc.identifier.eissn | 2005-7814 | - |
dc.identifier.pmid | 22053247 | - |
dc.subject.keyword | Anti-inflammation | - |
dc.subject.keyword | Heparin | - |
dc.subject.keyword | Osteoblastic function | - |
dc.subject.keyword | Titanium | - |
dc.subject.keyword | rhPDGF-BB | - |
dc.contributor.alternativeName | Shim, June Sung | - |
dc.contributor.affiliatedAuthor | Shim, June Sung | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 3 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 152 | - |
dc.citation.endPage | 160 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ADVANCED PROSTHODONTICS, Vol.3(3) : 152-160, 2011 | - |
dc.identifier.rimsid | 27774 | - |
dc.type.rims | ART | - |
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