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Vibrio vulnificus IlpA induces MAPK-mediated cytokine production via TLR1/2 activation in THP-1 cells, a human monocytic cell line

DC Field Value Language
dc.contributor.author박순정-
dc.date.accessioned2014-12-20T17:28:10Z-
dc.date.available2014-12-20T17:28:10Z-
dc.date.issued2011-
dc.identifier.issn0161-5890-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/94660-
dc.description.abstractVibrio vulnificus is a pathogenic bacterium causing primary septicemia, which is followed by a classical septic shock pathway including an overwhelming inflammatory cytokine response. V. vulnificus IlpA is a potent immunogenic lipoprotein that triggers cytokine production in human monocytes by activating the toll-like receptor 2 (TLR2). In this study, we further defined the IlpA signaling pathways involved in cytokine production in the human monocytic cell line, THP-1. TLR2 was involved in cytokine production by complexing with TLR1, but not with TLR6. MyD88 was necessary for IlpA-induced cytokine expression through TLR1/TLR2. Three mitogen activated protein kinases (MAPK), p38, ERK1/2, and JNK, were activated in THP-1 cells stimulated with recombinant IlpA (rIlpA). Selective inhibition of each MAPK resulted in significant decrease of rIlpA-induced cytokine production. Especially, functional TLR2 was necessary for IlpA-induced activation of p38 and JNK. IlpA augmented the DNA-binding activity of nuclear factor-kappaB (NF-κB) and activator protein-1 (AP-1) transcriptional factors to their recognition sites in THP-1 cells. These results suggest that serial activation of TLR1/TLR2, MyD88, the three MAPKs, and NF-κB/AP-1 comprises the signaling pathway responsible for proinflammatory cytokine production by V. vulnificus IlpA.-
dc.description.statementOfResponsibilityopen-
dc.format.extent143~154-
dc.relation.isPartOfMOLECULAR IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBacterial Proteins/immunology*-
dc.subject.MESHBacterial Proteins/metabolism-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Line-
dc.subject.MESHCytokines/biosynthesis-
dc.subject.MESHCytokines/immunology-
dc.subject.MESHElectrophoretic Mobility Shift Assay-
dc.subject.MESHFluorescent Antibody Technique-
dc.subject.MESHHumans-
dc.subject.MESHImmunoprecipitation-
dc.subject.MESHLipoproteins/immunology*-
dc.subject.MESHLipoproteins/metabolism-
dc.subject.MESHLipoproteins/physiology*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Knockout-
dc.subject.MESHMitogen-Activated Protein Kinases/immunology*-
dc.subject.MESHMitogen-Activated Protein Kinases/metabolism-
dc.subject.MESHMonocytes/immunology-
dc.subject.MESHMonocytes/metabolism-
dc.subject.MESHSignal Transduction/immunology-
dc.subject.MESHToll-Like Receptor 1/immunology*-
dc.subject.MESHToll-Like Receptor 1/metabolism-
dc.subject.MESHToll-Like Receptor 2/immunology*-
dc.subject.MESHToll-Like Receptor 2/metabolism-
dc.subject.MESHVibrio vulnificus/immunology-
dc.subject.MESHVibrio vulnificus/metabolism-
dc.titleVibrio vulnificus IlpA induces MAPK-mediated cytokine production via TLR1/2 activation in THP-1 cells, a human monocytic cell line-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorNa Yeon Lee-
dc.contributor.googleauthorHye-Yeon Lee-
dc.contributor.googleauthorKyu-Ho Lee-
dc.contributor.googleauthorSeung Hyun Han-
dc.contributor.googleauthorSoon-Jung Park-
dc.identifier.doi10.1016/j.molimm.2011.08.001-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01545-
dc.relation.journalcodeJ02259-
dc.identifier.eissn1872-9142-
dc.identifier.pmid21903273-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0161589011006924-
dc.subject.keywordIlp-
dc.subject.keywordAVibrio vulnificus-
dc.subject.keywordTHP-1 cell-
dc.subject.keywordMAP kinase-
dc.subject.keywordTranscription factor-
dc.contributor.alternativeNamePark, Soon Jung-
dc.contributor.affiliatedAuthorPark, Soon Jung-
dc.rights.accessRightsnot free-
dc.citation.volume49-
dc.citation.number1-2-
dc.citation.startPage143-
dc.citation.endPage154-
dc.identifier.bibliographicCitationMOLECULAR IMMUNOLOGY, Vol.49(1-2) : 143-154, 2011-
dc.identifier.rimsid27663-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

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