Cited 17 times in
Measurement of fluid secretion from intact airway submucosal glands
DC Field | Value | Language |
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dc.contributor.author | 최재영 | - |
dc.date.accessioned | 2014-12-20T17:25:12Z | - |
dc.date.available | 2014-12-20T17:25:12Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94568 | - |
dc.description.abstract | Human airways are kept sterile by a mucosal innate defense system that includes mucus secretion. Mucus is secreted in healthy upper airways primarily by submucosal glands and consists of defense molecules mixed with mucins, electrolytes, and water and is also a major component of sputum. Mucus traps pathogens and mechanically removes them via mucociliary clearance while inhibiting their growth via molecular (e.g., lysozyme) and cellular (e.g., neutrophils, macrophages) defenses. Fluid secretion rates of single glands in response to various mediators can be measured by trapping the primary gland mucus secretions in an oil layer, where they form spherical bubbles that can be optically measured at any desired interval to provide detailed temporal analysis of secretion rates. The composition and properties of the mucus (e.g., solids, viscosity, pH) can also be determined. These methods have now been applied to mice, ferrets, cats, pigs, sheep, and humans, with a main goal of comparing gland secretion in control and CFTR-deficient humans and animals | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | Methods in Molecular Biology (Clifton, N.J.) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Biological Transport | - |
dc.subject.MESH | Body Fluids/metabolism | - |
dc.subject.MESH | Cats | - |
dc.subject.MESH | Cystic Fibrosis/metabolism | - |
dc.subject.MESH | Cystic Fibrosis/physiopathology | - |
dc.subject.MESH | Cystic Fibrosis Transmembrane Conductance Regulator/metabolism | - |
dc.subject.MESH | Exocrine Glands/metabolism* | - |
dc.subject.MESH | Ferrets | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Microscopy | - |
dc.subject.MESH | Molecular Imaging/methods* | - |
dc.subject.MESH | Mucociliary Clearance* | - |
dc.subject.MESH | Mucous Membrane/metabolism | - |
dc.subject.MESH | Mucus*/chemistry | - |
dc.subject.MESH | Mucus*/metabolism | - |
dc.subject.MESH | Mucus*/secretion | - |
dc.subject.MESH | Respiratory Mucosa/cytology | - |
dc.subject.MESH | Respiratory Mucosa/metabolism* | - |
dc.subject.MESH | Sheep | - |
dc.subject.MESH | Species Specificity | - |
dc.subject.MESH | Sputum/metabolism | - |
dc.subject.MESH | Swine | - |
dc.subject.MESH | Trachea/cytology | - |
dc.subject.MESH | Trachea/metabolism | - |
dc.title | Measurement of fluid secretion from intact airway submucosal glands | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | Jeffrey J. Wine | - |
dc.contributor.googleauthor | Nam Soo Joo | - |
dc.contributor.googleauthor | Jae Young Choi | - |
dc.contributor.googleauthor | Hyung-Ju Cho | - |
dc.contributor.googleauthor | Mauri E. Krouse | - |
dc.contributor.googleauthor | Jin V. Wu | - |
dc.contributor.googleauthor | Monal Khansaheb | - |
dc.contributor.googleauthor | Toshiya Irokawa | - |
dc.contributor.googleauthor | Juan Ianowski | - |
dc.contributor.googleauthor | John W. Hanrahan | - |
dc.contributor.googleauthor | Alan W. Cuthbert | - |
dc.contributor.googleauthor | Kim V. Tran | - |
dc.identifier.doi | 10.1007/978-1-61779-120-8_6 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04173 | - |
dc.contributor.localId | A03936 | - |
dc.relation.journalcode | J02226 | - |
dc.identifier.pmid | 21547728 | - |
dc.identifier.url | http://link.springer.com/protocol/10.1007%2F978-1-61779-120-8_6 | - |
dc.subject.keyword | Submucosal gland | - |
dc.subject.keyword | exocrine secretion | - |
dc.subject.keyword | CFTR | - |
dc.subject.keyword | mucosal innate defense | - |
dc.subject.keyword | ion channel | - |
dc.subject.keyword | ion transporter | - |
dc.subject.keyword | calcium-activated chloride channel (CaCC) | - |
dc.subject.keyword | cAMP | - |
dc.subject.keyword | Ca2+ | - |
dc.subject.keyword | myoepithelial cell | - |
dc.subject.keyword | serous cell | - |
dc.subject.keyword | mucous cell | - |
dc.contributor.alternativeName | Choi, Jae Young | - |
dc.contributor.affiliatedAuthor | Choi, Jae Young | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 742 | - |
dc.citation.startPage | 93 | - |
dc.citation.endPage | 112 | - |
dc.identifier.bibliographicCitation | Methods in Molecular Biology (Clifton, N.J.), Vol.742 : 93-112, 2011 | - |
dc.identifier.rimsid | 27423 | - |
dc.type.rims | ART | - |
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