154 105

Cited 71 times in

Carbonic anhydrase IX (CA9) modulates tumor-associated cell migration and invasion

 Hye-Jin Shin  ;  Seung Bae Rho  ;  Dae Chul Jung  ;  Inn-Oc Han  ;  Eok-Soo Oh  ;  Joo-Young Kim 
 Journal of Cell Science, Vol.124(pt7) : 1077-1087, 2011 
Journal Title
 Journal of Cell Science 
Issue Date
Antigens, Neoplasm/genetics ; Antigens, Neoplasm/metabolism* ; Carbonic Anhydrase IX ; Carbonic Anhydrases/genetics ; Carbonic Anhydrases/metabolism* ; Cell Line, Tumor ; Cell Movement* ; Humans ; Neoplasm Metastasis* ; Neoplasms/enzymology* ; Neoplasms/genetics ; Neoplasms/pathology ; Neoplasms/physiopathology* ; Signal Transduction ; rho GTP-Binding Proteins/genetics ; rho GTP-Binding Proteins/metabolism
Carbonic anhydrase IX (CA9) ; Migration ; Invasion ; Cell adhesion ; Cytoskeleton ; Epithelial-mesenchymal transition (EMT) ; Rho ; Rho kinase
Expression of carbonic anhydrase IX (CA9) was shown to be strongly involved in high incidences of metastasis and poor prognosis in various human tumors. In this study, we investigated the possible role for CA9 in tumor metastases in vitro, using a gene transfection tool in the human cervical carcinoma cell line C33A. Gene expression profiling of CA9-transfected cells (C33A/CA9) and vector-transfected cells (C33A/Mock) was investigated by DNA microarray. The biological functions of differentially expressed genes between the C33A/CA9 and C33A/Mock cells included cell growth, regulation of cell-cell and cell-extracellular matrix adhesion and cytoskeletal organization. Immunofluorescent stain and Matrigel culture showed cytoskeletal remodeling, disassembled focal adhesion, weakened cell-cell adhesion and increased motility in C33A/CA9 cells. These invasive and metastatic phenotypes were associated with Rho-GTPase-related epithelial-mesenchymal transition. Inhibition of the Rho/Rho kinase pathway by a ROCK inhibitor (Y27632) and si-Rho (short interference RNA against RhoA) showed that Rho-GTPase signaling was involved in cellular morphologic and migratory changes. The effect of CA9 on Rho-GTPase signaling was also confirmed by silencing CA9 expression. Our results suggest that CA9 overexpression induces weakening of cell adhesions and augmented cell motility by aberrant Rho-GTPase signal transduction. Our study shows an underlying mechanism of CA9-related enhanced metastatic potential of tumor cells.
Files in This Item:
T201103888.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Jung, Dae Chul(정대철) ORCID logo https://orcid.org/0000-0001-5769-5083
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.