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Hoxc8 downregulates Mgl1 tumor suppressor gene expression and reduces its concomitant function on cell adhesion

 Kalyani Ruthala  ;  Jogeswar Gadi  ;  Myoung Hee Kim  ;  Hyun Joo Chung  ;  Heejei Yoon  ;  Ji-Yeon Lee 
 Molecules and Cells, Vol.32(3) : 273-279, 2011 
Journal Title
 Molecules and Cells 
Issue Date
Hoxc8 is a homeobox gene family member, which is essential for growth and differentiation. Mgl1, a mouse homologue of the Drosophila tumor suppressor gene lgl, was previously identified as a possible target of Hoxc8. However, the biological effects and underlying molecular mechanism of Hoxc8 regulation on Mgl1 has not been fully established. The endogenous expression patterns of Hoxc8 were inversely correlated with those of Mgl1 in different types of cells and tissues. Here we showed that Hoxc8 overexpression downregulated the Mgl1 mRNA expression. Characterization of the ~2 kb Mgl1 promoter region revealed that the upstream sequence contains several putative Hox core binding sites and chromatin immunoprecipitation assay confirmed that Hoxc8 directly binds to the 5' upstream region of Mgl1. The promoter activity of this region was diminished by Hoxc8 expression but resumed by knockdown of Hoxc8 using siRNA against Hoxc8. Functional study of Mgl1 in C3H10T1/2 cells revealed a significant reduction in cell adhesion upon expression of Hoxc8. Taken together, our data suggest that Hoxc8 downregulates Mgl1 expression via direct binding to the promoter region, which in turn reduces cell adhesion and concomitant cell migration.
Appears in Collections:
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Institute of Endocrinology (내분비연구소)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실)
Yonsei Authors
가디죠게스(Gadi, Jogeswar) ; 김명희(Kim, Myoung Hee) ; 이지연(Lee, Ji Yeon)
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