Cited 23 times in
Effect of hypoxia-inducible VEGF gene expression on revascularization and graft function in mouse islet transplantation.
DC Field | Value | Language |
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dc.contributor.author | 이병완 | - |
dc.date.accessioned | 2014-12-20T17:14:43Z | - |
dc.date.available | 2014-12-20T17:14:43Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0934-0874 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94231 | - |
dc.description.abstract | For gene transfer strategies to improve islet engraftment, vascular endothelial growth factor (VEGF) expression should be regulated in a way that matches the transient nature of revascularization with simultaneously avoiding undesirable effects of overexpression. The aim of this study was to investigate the effects of hypoxia-inducible VEGF gene transfer using the RTP801 promoter on islet grafts. We implanted pSV-hVEGF transfected, pRTP801-hVEGF transfected or nontransfected mouse islets under the kidney capsule of streptozotocin-induced diabetic syngeneic mice. Human VEGF immunostaining of day 3 grafts revealed that the pRTP801-hVEGF transfected group had higher hVEGF expression compared with the pSV-hVEGF transfected group. BS-1 staining of day 3 grafts from the pRTP801-hVEGF transfected group showed the highest vascular density, which was comparable with day 6 grafts from the nontransfected group. In 360 islet equivalent (IEQ)-transplantation which reverted hyperglycemia in all mice, the area under the curve of glucose levels during intraperitoneal glucose tolerance test 7 weeks post-transplant was lower in mice transplanted with pRTP801-hVEGF transfected grafts compared with mice transplanted with nontransfected grafts. In 220 IEQ-transplantations, diabetic mice transplanted with pRTP801-hVEGF islets became normoglycemic more rapidly compared with mice transplanted with pSV-hVEGF or nontransfected islets, and diabetes reversal rate after 50 days was 90%, 68%, and 50%, respectively. In conclusion, our results indicate that regulated overexpression of hVEGF in a hypoxia-inducible manner enhances islet vascular engraftment and preserves islet function overtime in transplants | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 307~314 | - |
dc.relation.isPartOf | TRANSPLANT INTERNATIONAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Diabetes Mellitus, Experimental/metabolism | - |
dc.subject.MESH | Genetic Therapy/methods | - |
dc.subject.MESH | Graft Survival | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypoxia/metabolism | - |
dc.subject.MESH | Islets of Langerhans/blood supply* | - |
dc.subject.MESH | Islets of Langerhans/physiology | - |
dc.subject.MESH | Islets of Langerhans Transplantation/methods | - |
dc.subject.MESH | Islets of Langerhans Transplantation/physiology* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Neovascularization, Physiologic/drug effects* | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/biosynthesis | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/physiology | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/therapeutic use* | - |
dc.title | Effect of hypoxia-inducible VEGF gene expression on revascularization and graft function in mouse islet transplantation. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Byung Wan Lee | - |
dc.contributor.googleauthor | Minhyung Lee | - |
dc.contributor.googleauthor | Hee Young Chae | - |
dc.contributor.googleauthor | Sanghyun Lee | - |
dc.contributor.googleauthor | Jun Goo Kang | - |
dc.contributor.googleauthor | Chul Sik Kim | - |
dc.contributor.googleauthor | Seong Jin Lee | - |
dc.contributor.googleauthor | Hyung Joon Yoo | - |
dc.contributor.googleauthor | Sung-Hee Ihm | - |
dc.identifier.doi | 10.1111/j.1432-2277.2010.01194.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02796 | - |
dc.relation.journalcode | J02753 | - |
dc.identifier.eissn | 1432-2277 | - |
dc.identifier.pmid | 21138485 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-2277.2010.01194.x/abstract | - |
dc.subject.keyword | hypoxia | - |
dc.subject.keyword | islet | - |
dc.subject.keyword | RTP801 | - |
dc.subject.keyword | transplantation | - |
dc.subject.keyword | vascular endothelial growth factor | - |
dc.contributor.alternativeName | Lee, Byung Wan | - |
dc.contributor.affiliatedAuthor | Lee, Byung Wan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 24 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 307 | - |
dc.citation.endPage | 314 | - |
dc.identifier.bibliographicCitation | TRANSPLANT INTERNATIONAL, Vol.24(3) : 307-314, 2011 | - |
dc.identifier.rimsid | 27478 | - |
dc.type.rims | ART | - |
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