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Cell death mediated by Vibrio parahaemolyticus type III secretion system 1 is dependent on ERK1/2 MAPK, but independent of caspases

DC Field Value Language
dc.contributor.author박순정-
dc.date.accessioned2014-12-20T17:11:49Z-
dc.date.available2014-12-20T17:11:49Z-
dc.date.issued2011-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/94140-
dc.description.abstractVibrio parahaemolyticus, which causes gastroenteritis, wound infection, and septicemia, has two sets of type III secretion systems (TTSS), TTSS1 and TTSS2. A TTSS1- deficient vcrD1 mutant of V. parahaemolyticus showed an attenuated cytotoxicity against HEp-2 cells, and a significant reduction in mouse lethality, which were both restored by complementation with the intact vcrD1 gene. V. parahaemolyticus also triggered phosphorylation of mitogenactivated protein kinases (MAPKs) including p38 and ERK1/2 in HEp-2 cells. The ability to activate p38 and ERK1/2 was significantly affected in a TTSS1-deficient vcrD1 mutant. Experiments using MAPK inhibitors showed that p38 and ERK1/2 MAPKs are involved in V. parahaemolyticus-induced death of HEp-2 cells. In addition, caspase-3 and caspase-9 were processed into active forms in V. parahaemolyticus-exposed HEp-2 cells, but activation of caspases was not essential for V. parahaemolyticusinduced death of HEp-2 cells, as shown by both annexin V staining and lactate dehydrogenase release assays. We conclude that secreted protein(s) of TTSS1 play an important role in activation of p38 and ERK1/2 in HEp-2 cells that eventually leads to cell death via a caspaseindependent mechanism.-
dc.description.statementOfResponsibilityopen-
dc.format.extent903~913-
dc.relation.isPartOfJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBacterial Proteins/genetics-
dc.subject.MESHBacterial Proteins/metabolism*-
dc.subject.MESHBacterialSecretionSystems*-
dc.subject.MESHCaspases/metabolism*-
dc.subject.MESHCellDeath-
dc.subject.MESHCellLine-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred ICR-
dc.subject.MESHMitogen-Activated Protein Kinase1/metabolism*-
dc.subject.MESHMitogen-Activated Protein Kinase 3/metabolism*-
dc.subject.MESHVibrioInfections/enzymology*-
dc.subject.MESHVibrioInfections/microbiology-
dc.subject.MESHVibrioInfections/physiopathology*-
dc.subject.MESHVibrioparahaemolyticus/genetics-
dc.subject.MESHVibrioparahaemolyticus/metabolism*-
dc.subject.MESHp38 Mitogen-Activated Protein Kinases/metabolism-
dc.titleCell death mediated by Vibrio parahaemolyticus type III secretion system 1 is dependent on ERK1/2 MAPK, but independent of caspases-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorYang, Yu Jin-
dc.contributor.googleauthorNa Kyung Lee-
dc.contributor.googleauthorNa Yeon Lee-
dc.contributor.googleauthorJong Woong Lee-
dc.contributor.googleauthorSoon-Jung Park-
dc.identifier.doi10.4014/jmb.1104.04044-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01545-
dc.relation.journalcodeJ01594-
dc.identifier.eissn1738-8872-
dc.identifier.pmid21952366-
dc.subject.keywordVibrio parahaemolyticus-
dc.subject.keywordmitogen-activated protein kinases-
dc.subject.keywordcaspases-
dc.contributor.alternativeNamePark, Soon Jung-
dc.contributor.affiliatedAuthorPark, Soon Jung-
dc.rights.accessRightsfree-
dc.citation.volume21-
dc.citation.number9-
dc.citation.startPage903-
dc.citation.endPage913-
dc.identifier.bibliographicCitationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Vol.21(9) : 903-913, 2011-
dc.identifier.rimsid27266-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

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