Cited 4 times in
Cell death mediated by Vibrio parahaemolyticus type III secretion system 1 is dependent on ERK1/2 MAPK, but independent of caspases
DC Field | Value | Language |
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dc.contributor.author | 박순정 | - |
dc.date.accessioned | 2014-12-20T17:11:49Z | - |
dc.date.available | 2014-12-20T17:11:49Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94140 | - |
dc.description.abstract | Vibrio parahaemolyticus, which causes gastroenteritis, wound infection, and septicemia, has two sets of type III secretion systems (TTSS), TTSS1 and TTSS2. A TTSS1- deficient vcrD1 mutant of V. parahaemolyticus showed an attenuated cytotoxicity against HEp-2 cells, and a significant reduction in mouse lethality, which were both restored by complementation with the intact vcrD1 gene. V. parahaemolyticus also triggered phosphorylation of mitogenactivated protein kinases (MAPKs) including p38 and ERK1/2 in HEp-2 cells. The ability to activate p38 and ERK1/2 was significantly affected in a TTSS1-deficient vcrD1 mutant. Experiments using MAPK inhibitors showed that p38 and ERK1/2 MAPKs are involved in V. parahaemolyticus-induced death of HEp-2 cells. In addition, caspase-3 and caspase-9 were processed into active forms in V. parahaemolyticus-exposed HEp-2 cells, but activation of caspases was not essential for V. parahaemolyticusinduced death of HEp-2 cells, as shown by both annexin V staining and lactate dehydrogenase release assays. We conclude that secreted protein(s) of TTSS1 play an important role in activation of p38 and ERK1/2 in HEp-2 cells that eventually leads to cell death via a caspaseindependent mechanism. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 903~913 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Bacterial Proteins/genetics | - |
dc.subject.MESH | Bacterial Proteins/metabolism* | - |
dc.subject.MESH | BacterialSecretionSystems* | - |
dc.subject.MESH | Caspases/metabolism* | - |
dc.subject.MESH | CellDeath | - |
dc.subject.MESH | CellLine | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred ICR | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase1/metabolism* | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3/metabolism* | - |
dc.subject.MESH | VibrioInfections/enzymology* | - |
dc.subject.MESH | VibrioInfections/microbiology | - |
dc.subject.MESH | VibrioInfections/physiopathology* | - |
dc.subject.MESH | Vibrioparahaemolyticus/genetics | - |
dc.subject.MESH | Vibrioparahaemolyticus/metabolism* | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/metabolism | - |
dc.title | Cell death mediated by Vibrio parahaemolyticus type III secretion system 1 is dependent on ERK1/2 MAPK, but independent of caspases | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Yang, Yu Jin | - |
dc.contributor.googleauthor | Na Kyung Lee | - |
dc.contributor.googleauthor | Na Yeon Lee | - |
dc.contributor.googleauthor | Jong Woong Lee | - |
dc.contributor.googleauthor | Soon-Jung Park | - |
dc.identifier.doi | 10.4014/jmb.1104.04044 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01545 | - |
dc.relation.journalcode | J01594 | - |
dc.identifier.eissn | 1738-8872 | - |
dc.identifier.pmid | 21952366 | - |
dc.subject.keyword | Vibrio parahaemolyticus | - |
dc.subject.keyword | mitogen-activated protein kinases | - |
dc.subject.keyword | caspases | - |
dc.contributor.alternativeName | Park, Soon Jung | - |
dc.contributor.affiliatedAuthor | Park, Soon Jung | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 21 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 903 | - |
dc.citation.endPage | 913 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Vol.21(9) : 903-913, 2011 | - |
dc.identifier.rimsid | 27266 | - |
dc.type.rims | ART | - |
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