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Molecular identification of Taenia tapeworms by Cox1 gene in Koh Kong, Cambodia

Authors
 Hyeong-Kyu Jeon  ;  Tai-Soon Yong  ;  Woon-Mok Sohn  ;  Jong-Yil Chai  ;  Sung-Jong Hong  ;  Eun-Taek Han  ;  Hoo-Gn Jeong  ;  Tep Chhakda  ;  Muth Sinuon  ;  Duong Socheat  ;  Keeseon S. Eom 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.49(2) : 195-197, 2011 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
2011
MeSH
Adolescent ; Adult ; Animals ; Cambodia ; Child ; Cyclooxygenase 1/genetics* ; DNA Primers/genetics ; DNA, Helminth/chemistry ; DNA, Helminth/genetics ; Feces/parasitology ; Female ; Helminth Proteins/genetics* ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Taeniasaginata/enzymology* ; Taeniasaginata/genetics* ; Taeniasaginata/isolation & purification ; Taeniasolium/enzymology* ; Taeniasolium/genetics* ; Taeniasolium/isolation & purification ; Taeniasis/parasitology* ; Young Adult
Keywords
Taenia saginata ; Taenia solium ; molecular survey ; egg DNA ; multiplex PCR
Abstract
We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.
Files in This Item:
T201103273.pdf Download
DOI
10.3347/kjp.2011.49.2.195
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94124
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