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Molecular identification of Taenia tapeworms by Cox1 gene in Koh Kong, Cambodia

Authors
 Hyeong-Kyu Jeon ; Tai-Soon Yong ; Keeseon S. Eom ; Duong Socheat ; Muth Sinuon ; Tep Chhakda ; Hoo-Gn Jeong ; Eun-Taek Han ; Sung-Jong Hong ; Jong-Yil Chai ; Woon-Mok Sohn 
Citation
 Korean Journal of Parasitology, Vol.49(2) : 195~197, 2011 
Journal Title
 Korean Journal of Parasitology 
ISSN
 0023-4001 
Issue Date
2011
Abstract
We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94124
DOI
10.3347/kjp.2011.49.2.195
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Environmental Medical Biology
Yonsei Authors
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