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Identification of oxidized serum albumin in the cerebrospinal fluid of ischaemic stroke patients

Authors
 G. J. Moon  ;  D. H. Shin  ;  D. S. Im  ;  O. Y. Bang  ;  H. S. Nam  ;  J. H. Lee  ;  I. S. Joo  ;  K. Huh  ;  B. J. Gwag 
Citation
 European Journal of Neurology, Vol.18(9) : 1151-1158, 2011 
Journal Title
 European Journal of Neurology 
ISSN
 1351-5101 
Issue Date
2011
Abstract
BACKGROUND AND PURPOSE:   Extensive evidence has shown that oxidative stress mediates neuronal death in animal models of hypoxic-ischaemia. Brain biomarkers of oxidative stress need to be identified in order to better understand and treat brain damage in human stroke patients. The present study was conducted to identify potential target proteins of oxidative stress in the cerebrospinal fluid (CSF) of stroke patients with acute ischaemic brain injury. METHODS:   We performed two-dimensional polyacrylamide gel electrophoresis to separate protein samples obtained from the CSF of control and stroke patients. To determine protein oxidation levels, oxyblot was then used to detect protein carbonyls that were determined by formation of a stable 2,4-dinitrophenylhydrazine (DNP) product using an anti-DNP antibody. RESULTS:   We found that oxidation of serum albumin was increased in the CSF from stroke patients as well as rats who underwent permanent middle cerebral artery occlusion (6.5%, 23%, respectively). In stroke patients, oxidized albumin levels correlated to neurologic indications. CONCLUSIONS:   The present study suggests that oxidized albumin in CSF can be utilized as an oxidative stress marker in human stroke patients
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93984
DOI
10.1111/j.1468-1331.2011.03357.x
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실)
Yonsei Authors
남효석(Nam, Hyo Suk)
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Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1468-1331.2011.03357.x/abstract
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