3 235

Cited 128 times in

Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations.

Authors
 Jung-Chul Park  ;  Jeong-Min Kim  ;  Im-Hee Jung  ;  Jane C. Kim  ;  Seong-Ho Choi  ;  Kyoo-Sung Cho  ;  Chang-Sung Kim 
Citation
 JOURNAL OF CLINICAL PERIODONTOLOGY, Vol.38(8) : 721-731, 2011 
Journal Title
 JOURNAL OF CLINICAL PERIODONTOLOGY 
ISSN
 0303-6979 
Issue Date
2011
MeSH
Aged ; Chemokines/blood* ; Female ; Humans ; Inflammation Mediators/blood* ; Insulin Resistance* ; Metabolic Syndrome/blood* ; Middle Aged ; Risk Reduction Behavior*
Abstract
OBJECTIVES: Mesenchymal stem cells (MSC) could be isolated from healthy periodontal ligaments (PDL). The aims of this study were to isolate and characterize human PDL stem cells (hPDLSCs) from inflamed PDL tissue, and to evaluate their regenerative potential. MATERIALS AND METHODS: Inflamed hPDLSCs (ihPDLSCs) were isolated from the inflamed PDL tissue obtained from intra-bony defects during flap surgery, and characterized by immunohistochemical staining, colony-forming unit assay, fluorescence-activated cell sorting, and mRNA expression in comparison with healthy hPDLSCs obtained from extracted teeth for orthodontic purpose. The proliferative potential and migratory potential was evaluated, and compared with healthy hPDLSCs. Regenerative potential was assessed by an in vivo ectopic transplantation model. RESULTS: ihPDLSCs were successfully isolated and characterized as MSCs. Both ihPDLSCs and hPDLSCs were successfully differentiated under osteogenic/cementogenic and adipogenic microenvironment. The proliferative potential did not differ between healthy hPDLSCs and ihPDLSCs, while the migratory capacity was significantly increased in ihPDLSCs (p<0.05). Both groups exhibited new cementum-like tissue and related PDL fibre regeneration in an in vivo transplantation model. CONCLUSION: ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-051X.2011.01716.x/abstract
DOI
10.1111/j.1600-051X.2011.01716.x
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jeong Min(김정민)
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Park, Jung Chul(박정철)
Jung, Im Hee(정임희)
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93576
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse