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Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations.

Authors
 Jung-Chul Park  ;  Jeong-Min Kim  ;  Chang-Sung Kim  ;  Kyoo-Sung Cho  ;  Seong-Ho Choi  ;  Jane C. Kim  ;  Im-Hee Jung 
Citation
 Journal of Clinical Periodontology, Vol.38(8) : 721-731, 2011 
Journal Title
 Journal of Clinical Periodontology 
ISSN
 0303-6979 
Issue Date
2011
Abstract
OBJECTIVES: Mesenchymal stem cells (MSC) could be isolated from healthy periodontal ligaments (PDL). The aims of this study were to isolate and characterize human PDL stem cells (hPDLSCs) from inflamed PDL tissue, and to evaluate their regenerative potential. MATERIALS AND METHODS: Inflamed hPDLSCs (ihPDLSCs) were isolated from the inflamed PDL tissue obtained from intra-bony defects during flap surgery, and characterized by immunohistochemical staining, colony-forming unit assay, fluorescence-activated cell sorting, and mRNA expression in comparison with healthy hPDLSCs obtained from extracted teeth for orthodontic purpose. The proliferative potential and migratory potential was evaluated, and compared with healthy hPDLSCs. Regenerative potential was assessed by an in vivo ectopic transplantation model. RESULTS: ihPDLSCs were successfully isolated and characterized as MSCs. Both ihPDLSCs and hPDLSCs were successfully differentiated under osteogenic/cementogenic and adipogenic microenvironment. The proliferative potential did not differ between healthy hPDLSCs and ihPDLSCs, while the migratory capacity was significantly increased in ihPDLSCs (p<0.05). Both groups exhibited new cementum-like tissue and related PDL fibre regeneration in an in vivo transplantation model. CONCLUSION: ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93576
DOI
10.1111/j.1600-051X.2011.01716.x
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Periodontology (치주과학교실)
Yonsei Authors
김정민(Kim, Jeong Min) ; 김창성(Kim, Chang Sung) ; 박정철(Park, Jung Chul) ; 정임희(Jung, Im Hee) ; 조규성(Cho, Kyoo Sung) ; 최성호(Choi, Seong Ho)
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Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-051X.2011.01716.x/abstract
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