Cited 8 times in
Calpain mediates degradation of cytoskeletal proteins during Jurkat T-cell death induced by Entamoeba histolytica
DC Field | Value | Language |
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dc.contributor.author | 김경아 | - |
dc.contributor.author | 신명헌 | - |
dc.contributor.author | 이영아 | - |
dc.date.accessioned | 2014-12-20T16:44:42Z | - |
dc.date.available | 2014-12-20T16:44:42Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0141-9838 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93296 | - |
dc.description.abstract | Entamoeba histolytica is known to induce host cell death via activation of calpain and caspases. In this study, we investigated the specific proteases involved in the degradation of cytoskeletal proteins during Jurkat T-cell death induced by E. histolytica. Amoebic trophozoites induced marked degradation of paxillin, Cas, vimentin, vinculin and talin, as well as α- or β-spectrin, in Jurkat T cells. The cleavage effects of E. histolytica were strongly retarded by pretreatment with a calpain inhibitor, but not with a pan-caspase inhibitor. In addition, calpain knockdown with siRNA in Jurkat T cells effectively inhibited E. histolytica-induced PARP, paxillin, α-spectrin, β-spectrin and talin degradation, as compared to scrambled siRNA. These results suggest that calpain plays a crucial role in the cleavage of cytoskeletal proteins during cell death induced by E. histolytica. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 349~356 | - |
dc.relation.isPartOf | PARASITE IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Calpain/metabolism* | - |
dc.subject.MESH | Cell Death* | - |
dc.subject.MESH | Cytoskeletal Proteins/metabolism* | - |
dc.subject.MESH | Entamoeba histolytica/immunology* | - |
dc.subject.MESH | Entamoeba histolytica/pathogenicity* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Jurkat Cells/metabolism* | - |
dc.title | Calpain mediates degradation of cytoskeletal proteins during Jurkat T-cell death induced by Entamoeba histolytica | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Y. A. LEE | - |
dc.contributor.googleauthor | K. A. KIM | - |
dc.contributor.googleauthor | M. H. SHIN | - |
dc.identifier.doi | 10.1111/j.1365-3024.2011.01290.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02099 | - |
dc.contributor.localId | A02959 | - |
dc.contributor.localId | A00300 | - |
dc.relation.journalcode | J02466 | - |
dc.identifier.eissn | 1365-3024 | - |
dc.identifier.pmid | 21426360 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3024.2011.01290.x/abstract | - |
dc.subject.keyword | Calpain | - |
dc.subject.keyword | Cytoskeleton proteins | - |
dc.subject.keyword | Entamoeba histolytica | - |
dc.subject.keyword | Host cell death | - |
dc.contributor.alternativeName | Kim, Kyung Ah | - |
dc.contributor.alternativeName | Shin, Myeong Heon | - |
dc.contributor.alternativeName | Lee, Young Ah | - |
dc.contributor.affiliatedAuthor | Shin, Myeong Heon | - |
dc.contributor.affiliatedAuthor | Lee, Young Ah | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Ah | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 33 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 349 | - |
dc.citation.endPage | 356 | - |
dc.identifier.bibliographicCitation | PARASITE IMMUNOLOGY, Vol.33(6) : 349-356, 2011 | - |
dc.identifier.rimsid | 27117 | - |
dc.type.rims | ART | - |
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