Cited 24 times in
Toll-like receptor 4 signaling is involved in IgA-stimulated mesangial cell activation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임범진 | - |
dc.contributor.author | 정현주 | - |
dc.contributor.author | 홍순원 | - |
dc.date.accessioned | 2014-12-20T16:43:40Z | - |
dc.date.available | 2014-12-20T16:43:40Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93263 | - |
dc.description.abstract | PURPOSE: Deposition of polymeric IgA1 in the kidney mesangium is the hallmark of IgA nephropathy, but the molecular mechanisms of IgA-mediated mesangial responses and inflammatory injuries remain poorly understood. We hypothesize that Toll-like receptor 4 (TLR4) is involved in IgA-induced mesangial cell activation. MATERIALS AND METHODS: Mouse mesangial cells were stimulated with lipopolysaccharide (LPS) (1 μg/mL), IgA (20 μg/mL), or both, and TLR4 expression was measured by real time RT-PCR and Western blot. Intracellular responses to LPS or IgA were assessed by Western blot for ERK1/2, JNK, p38 MAP kinases (MAPKs), Iκ-Bα degradation and fibronectin secretion. MCP-1 secretion was assessed by ELISA. Small interfering RNA (siRNA) of TLR4 was used to confirm that the effects were caused by TLR4 activity. RESULTS: LPS- or IgA-treatment upregulated the levels of TLR4 mRNA and protein in cultured MMC at 24 h. LPS and IgA induced rapid phosphorylation of MAPKs, but degradation of Iκ-Bα was observed only in LPS-treated MMC. LPS, but not IgA, induced increased secretion of MCP-1 and fibronectin at 24 h or 48 h. Combined LPS and IgA treatment did not cause additional increases in TLR4 mRNA and protein levels or Iκ-Bα degradation, and MCP-1 and fibronectin secretions were less than with LPS alone. LPS- or IgA-induced TLR4 protein levels and MAPK activation were inhibited by transfection with TLR4 siRNA. CONCLUSION: These results indicate that the activation of MAPKs and MCP-1 secretion are mediated by TLR4, at least in part, in IgA-treated mesangial cells. TLR4 is involved in mesangial cell injury by induction of pro-inflammatory cytokines in IgA nephropathy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 610~615 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Chemokine CCL2/secretion | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/metabolism | - |
dc.subject.MESH | Fibronectins/secretion | - |
dc.subject.MESH | Glomerulonephritis, IGA/metabolism* | - |
dc.subject.MESH | I-kappa B Proteins/metabolism | - |
dc.subject.MESH | Mesangial Cells/metabolism* | - |
dc.subject.MESH | Mesangial Cells/secretion | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | NF-KappaB Inhibitor alpha | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | RNA Interference | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Signal Transduction* | - |
dc.subject.MESH | Toll-Like Receptor 4/antagonists & inhibitors | - |
dc.subject.MESH | Toll-Like Receptor 4/genetics | - |
dc.subject.MESH | Toll-Like Receptor 4/metabolism* | - |
dc.title | Toll-like receptor 4 signaling is involved in IgA-stimulated mesangial cell activation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Beom Jin Lim | - |
dc.contributor.googleauthor | Dahye Lee | - |
dc.contributor.googleauthor | Soon Won Hong | - |
dc.contributor.googleauthor | Hyeon Joo Jeong | - |
dc.identifier.doi | 10.3349/ymj.2011.52.4.610 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03363 | - |
dc.contributor.localId | A03771 | - |
dc.contributor.localId | A04411 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 21623603 | - |
dc.subject.keyword | IgA nephropathy | - |
dc.subject.keyword | mesangial cell | - |
dc.subject.keyword | cytokine | - |
dc.subject.keyword | toll-like receptor | - |
dc.contributor.alternativeName | Lim, Beom Jin | - |
dc.contributor.alternativeName | Jeong, Hyeon Joo | - |
dc.contributor.alternativeName | Hong, Soon Won | - |
dc.contributor.affiliatedAuthor | Lim, Beom Jin | - |
dc.contributor.affiliatedAuthor | Jeong, Hyeon Joo | - |
dc.contributor.affiliatedAuthor | Hong, Soon Won | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 52 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 610 | - |
dc.citation.endPage | 615 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.52(4) : 610-615, 2011 | - |
dc.identifier.rimsid | 27101 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.