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Molecular beacon-based bioimaging of multiple microRNAs during myogenesis.

Authors
 Won Jun Kang ; Ye Lim Cho ; Soonhag Kim ; Kyung-Ju Choi ; Jong Doo Lee ; Ju Ri Chae 
Citation
 Biomaterials, Vol.32(7) : 1915~1922, 2011 
Journal Title
 Biomaterials 
ISSN
 0142-9612 
Issue Date
2011
Abstract
MicroRNAs (miRNAs, miR) are associated with multiple cellular processes and diseases. Here, we designed fluorescent DNA probes composed of stem loop-structured DNA complementary to miRNAs and fluorophore-quencher pairs [molecular beacon (MB)] to simultaneously monitor the biogenesis of miR-206 and miR-26a, which are highly expressed during myogenic differentiation. C2C12 cells were transfected with an MB targeting miR-26a and containing a 6-FAM-BHQ1 pair (miRNA-26a MB) or an MB targeting miR-206 with a Texas Red-BHQ2 pair (miRNA-206 MB). In vitro and in vivo fluorescence analysis revealed that, only in differentiated single C2C12 cell, significantly increased fluorescence signals of miRNA-26a MB, miRNA-206 MB or simultaneous incubation of both beacons were detected due to the hybridization of miR-206 or miR-26a with their respective beacons, resulting in activation of fluorescence. Our MB-based miRNA imaging system may serve as a new imaging probe for monitoring multiple miRNAs during various cellular or disease processes associated with miRNAs.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/92965
DOI
10.1016/j.biomaterials.2010.11.007
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Nuclear Medicine
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0142961210014298
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