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Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation (MOFF) and dielectrophoresis (DEP).

Authors
 Hui-Sung Moon  ;  Kiho Kwon  ;  Seung-Il Kim  ;  Hyunju Han  ;  Joohyuk Sohn  ;  Soohyeon Lee  ;  Hyo-Il Jung 
Citation
 Lab on a Chip, Vol.11(6) : 1118-1125, 2011 
Journal Title
 Lab on a Chip 
ISSN
 1473-0197 
Issue Date
2011
Abstract
Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer, so their isolations and quantifications are important for biomedical applications such as cancer prognosis and measuring the responses to drug treatments. In this paper, we present the development of a microfluidic device for the separation of CTCs from blood cells based on the physical properties of cells. For use as a CTC model, we successfully separated human breast cancer cells (MCF-7) from a spiked blood cell sample by combining multi-orifice flow fractionation (MOFF) and dielectrophoretic (DEP) cell separation technique. Hydrodynamic separation takes advantage of the massive and high-throughput filtration of blood cells as it can accommodate a very high flow rate. DEP separation plays a role in precise post-processing to enhance the efficiency of the separation. The serial combination of these two different sorting techniques enabled high-speed continuous flow-through separation without labeling. We observed up to a 162-fold increase in MCF-7 cells at a 126 µL min(-1) flow rate. Red and white blood cells were efficiently removed with separation efficiencies of 99.24% and 94.23% respectively. Therefore, we suggest that our system could be used for separation and detection of CTCs from blood cells for biomedical applications.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/92618
DOI
10.1039/c0lc00345j
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
Yonsei Authors
김승일(Kim, Seung Il) ; 손주혁(Sohn, Joo Hyuk)
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Full Text
http://dx.doi.org/10.1039/c0lc00345j
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