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Induction of pulmonary mucosal immune responses with a protein vaccine targeted to the DEC-205/CD205 receptor

 Yoonkyung Do  ;  Arnaud M. Didierlaurent  ;  Seongho Ryu  ;  Hyein Koh  ;  Chae Gyu Park  ;  Steven Park  ;  David S. Perlin  ;  Bradford S. Powell  ;  Ralph M. Steinman 
 VACCINE, Vol.30(45) : 6359-6367, 2012 
Journal Title
Issue Date
Adjuvants, Immunologic/pharmacology ; Administration, Intranasal ; Animals ; Antibodies, Bacterial/immunology ; Antigens, Bacterial/immunology ; Antigens, CD/immunology* ; CD4-Positive T-Lymphocytes/immunology ; Dendritic Cells/immunology* ; Immunity, Cellular ; Immunity, Humoral ; Immunity, Mucosal* ; Immunoglobulin A/immunology ; Immunoglobulin G/immunology ; Interferon-gamma/immunology ; Lectins, C-Type/immunology* ; Lung/immunology* ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C3H ; Mice, Inbred C57BL ; Minor Histocompatibility Antigens ; Plague/prevention & control* ; Plague Vaccine/immunology ; Poly I-C/immunology ; Poly I-C/pharmacology ; Pore Forming Cytotoxic Proteins/immunology ; Receptors, Cell Surface/immunology* ; Yersinia pestis/pathogenicity
Dendritic cells ; CD205/DEC-205 ; Y. pestis ; LcrV ; Cellular immunity ; Mucosal
It is of great interest to develop a pneumonic plague vaccine that would induce combined humoral and cellular immunity in the lung. Here we investigate a novel approach based on targeting of dendritic cells using the DEC-205/CD205 receptor (DEC) via the intranasal route as way to improve mucosal cellular immunity to the vaccine. Intranasal administration of Yersinia pestis LcrV (V) protein fused to anti-DEC antibody together with poly IC as an adjuvant induced high frequencies of IFN-γ secreting CD4+ T cells in the airway and lung as well as pulmonary IgG and IgA antibodies. Anti-DEC:LcrV was more efficient to induce IFN-γ/TNF-α/IL-2 secreting polyfunctional CD4+ T cells when compared to non-targeted soluble protein vaccine. In addition, the intranasal route of immunization with anti-DEC:LcrV was associated with improved survival upon pulmonary challenge with the virulent CO92 Y. pestis. Taken together, these data indicate that targeting dendritic cells via the mucosal route is a potential new avenue for the development of a mucosal vaccine against pneumonic plague.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
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