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Decreased lung hyaluronan in a model of ARDS in the rat: effect of an inhibitor of leukocyte elastase

DC FieldValueLanguage
dc.contributor.author안철민-
dc.date.accessioned2014-12-19T17:45:20Z-
dc.date.available2014-12-19T17:45:20Z-
dc.date.issued2012-
dc.identifier.issn0300-9734-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91995-
dc.description.abstractBACKGROUND: Hyaluronan (HA) is a component of the extracellular matrix in lung tissue and is normally present at low concentrations in blood. HA is rapidly cleared from blood by the liver. Increased concentrations of plasma HA have been found in patients with acute respiratory distress syndrome (ARDS). We investigated changes in HA levels in plasma, bronchoalveolar lavage fluid (BALF), and lung, and their relationship to pretreatment with a leukocyte elastase inhibitor in a rat model of ARDS. METHODS: Rats were randomly assigned to three groups: control, thrombin, and thrombin plus elastase inhibitor. By use of a radiometric assay, HA was measured in lungs, BALF, and plasma. Tissue samples from the lungs were stained for HA and examined microscopically. Liver circulation and cardiac output were monitored using radiolabeled microspheres. RESULTS: Infusion of thrombin produced a pronounced increase in wet weight to dry weight ratio, and relative lung water content. This increase was blunted by a leukocyte elastase inhibitor. A decrease in lung HA and increases in both BALF and plasma HA were found. The leukocyte elastase inhibitor counteracted not only the decrease in lung tissue HA, but also the increase in plasma HA. Histologically, there was decreased HA-staining of peribronchial and perivascular areas in the injured rat lung. Decreased liver perfusion was observed after infusion of thrombin. CONCLUSIONS: The decrease in lung HA may be involved in the development of pulmonary edema in this ARDS model, and leukocyte elastase may be one cause of this decrease. In addition, an elevated plasma HA level may be an indicator of lung injury.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfUPSALA JOURNAL OF MEDICAL SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBronchoalveolar Lavage Fluid-
dc.subject.MESHDisease Models, Animal*-
dc.subject.MESHEnzyme Inhibitors/pharmacology*-
dc.subject.MESHHyaluronic Acid/blood-
dc.subject.MESHHyaluronic Acid/metabolism*-
dc.subject.MESHLeukocyte Elastase/antagonists & inhibitors*-
dc.subject.MESHLung/enzymology-
dc.subject.MESHLung/metabolism*-
dc.subject.MESHMale-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHRespiratory Distress Syndrome, Adult/enzymology-
dc.subject.MESHRespiratory Distress Syndrome, Adult/metabolism*-
dc.titleDecreased lung hyaluronan in a model of ARDS in the rat: effect of an inhibitor of leukocyte elastase-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorCHUL MIN AHN-
dc.contributor.googleauthorHÅKAN SANDLER-
dc.contributor.googleauthorTOM SALDEEN-
dc.identifier.doi22283425-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02269-
dc.relation.journalcodeJ02772-
dc.identifier.eissn2000-1967-
dc.identifier.pmid22283425-
dc.subject.keywordAcute lung injury-
dc.subject.keywordacute respiratory distress syndrome-
dc.subject.keywordhyaluronan-
dc.subject.keywordleukocyte elastase inhibitor-
dc.subject.keywordpulmonary edema-
dc.contributor.alternativeNameAhn, Chul Min-
dc.contributor.affiliatedAuthorAhn, Chul Min-
dc.citation.volume117-
dc.citation.number1-
dc.citation.startPage1-
dc.citation.endPage9-
dc.identifier.bibliographicCitationUPSALA JOURNAL OF MEDICAL SCIENCES, Vol.117(1) : 1-9, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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