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Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김순일 | - |
dc.contributor.author | 김유선 | - |
dc.contributor.author | 김현숙 | - |
dc.date.accessioned | 2014-12-19T17:43:21Z | - |
dc.date.available | 2014-12-19T17:43:21Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0001-2815 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931 | - |
dc.description.abstract | The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | TISSUE ANTIGENS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Alleles* | - |
dc.subject.MESH | Behcet Syndrome/diagnosis* | - |
dc.subject.MESH | Behcet Syndrome/genetics* | - |
dc.subject.MESH | Behcet Syndrome/immunology | - |
dc.subject.MESH | Genotype | - |
dc.subject.MESH | HLA-B51 Antigen/genetics* | - |
dc.subject.MESH | HLA-B51 Antigen/immunology | - |
dc.subject.MESH | Histocompatibility Testing/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Sequence Analysis, DNA | - |
dc.title | Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Y. Park | - |
dc.contributor.googleauthor | Y. S. Kim | - |
dc.contributor.googleauthor | S. I. Kim | - |
dc.contributor.googleauthor | H. Kim | - |
dc.contributor.googleauthor | H. S. Kim | - |
dc.identifier.doi | 22861687 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00649 | - |
dc.contributor.localId | A00785 | - |
dc.contributor.localId | A01117 | - |
dc.relation.journalcode | J02731 | - |
dc.identifier.eissn | 1399-0039 | - |
dc.identifier.pmid | 22861687 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1399-0039.2012.01942.x/abstract | - |
dc.subject.keyword | Behcet’s disease | - |
dc.subject.keyword | human leukocyteantigen-B51 | - |
dc.subject.keyword | real-time polymerase chainreaction | - |
dc.subject.keyword | sequence-based typing | - |
dc.subject.keyword | sequence-specific priming polymerasechain reactio | - |
dc.contributor.alternativeName | Kim, Soon Il | - |
dc.contributor.alternativeName | Kim, Yu Seun | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.affiliatedAuthor | Kim, Soon Il | - |
dc.contributor.affiliatedAuthor | Kim, Yu Seun | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.citation.volume | 80 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 376 | - |
dc.citation.endPage | 379 | - |
dc.identifier.bibliographicCitation | TISSUE ANTIGENS , Vol.80(4) : 376-379, 2012 | - |
dc.identifier.rimsid | 30013 | - |
dc.type.rims | ART | - |
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