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Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing

DC FieldValueLanguage
dc.contributor.author김순일-
dc.contributor.author김유선-
dc.contributor.author김현숙-
dc.date.accessioned2014-12-19T17:43:21Z-
dc.date.available2014-12-19T17:43:21Z-
dc.date.issued2012-
dc.identifier.issn0001-2815-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91931-
dc.description.abstractThe human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfTISSUE ANTIGENS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAlleles*-
dc.subject.MESHBehcet Syndrome/diagnosis*-
dc.subject.MESHBehcet Syndrome/genetics*-
dc.subject.MESHBehcet Syndrome/immunology-
dc.subject.MESHGenotype-
dc.subject.MESHHLA-B51 Antigen/genetics*-
dc.subject.MESHHLA-B51 Antigen/immunology-
dc.subject.MESHHistocompatibility Testing/methods*-
dc.subject.MESHHumans-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHSensitivity and Specificity-
dc.subject.MESHSequence Analysis, DNA-
dc.titleEvaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorY. Park-
dc.contributor.googleauthorY. S. Kim-
dc.contributor.googleauthorS. I. Kim-
dc.contributor.googleauthorH. Kim-
dc.contributor.googleauthorH. S. Kim-
dc.identifier.doi22861687-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00649-
dc.contributor.localIdA00785-
dc.contributor.localIdA01117-
dc.relation.journalcodeJ02731-
dc.identifier.eissn1399-0039-
dc.identifier.pmid22861687-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1399-0039.2012.01942.x/abstract-
dc.subject.keywordBehcet’s disease-
dc.subject.keywordhuman leukocyteantigen-B51-
dc.subject.keywordreal-time polymerase chainreaction-
dc.subject.keywordsequence-based typing-
dc.subject.keywordsequence-specific priming polymerasechain reactio-
dc.contributor.alternativeNameKim, Soon Il-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameKim, Hyon Suk-
dc.contributor.affiliatedAuthorKim, Soon Il-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorKim, Hyon Suk-
dc.citation.volume80-
dc.citation.number4-
dc.citation.startPage376-
dc.citation.endPage379-
dc.identifier.bibliographicCitationTISSUE ANTIGENS , Vol.80(4) : 376-379, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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