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Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing

Authors
 Y. Park  ;  Y. S. Kim  ;  S. I. Kim  ;  H. Kim  ;  H. S. Kim 
Citation
 TISSUE ANTIGENS , Vol.80(4) : 376-379, 2012 
Journal Title
 TISSUE ANTIGENS 
ISSN
 0001-2815 
Issue Date
2012
MeSH
Alleles* ; Behcet Syndrome/diagnosis* ; Behcet Syndrome/genetics* ; Behcet Syndrome/immunology ; Genotype ; HLA-B51 Antigen/genetics* ; HLA-B51 Antigen/immunology ; Histocompatibility Testing/methods* ; Humans ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Sequence Analysis, DNA
Keywords
Behcet’s disease ; human leukocyteantigen-B51 ; real-time polymerase chainreaction ; sequence-based typing ; sequence-specific priming polymerasechain reactio
Abstract
The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1399-0039.2012.01942.x/abstract
DOI
22861687
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Soon Il(김순일) ORCID logo https://orcid.org/0000-0002-0783-7538
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931
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