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miR-449a regulates the chondrogenesis of human mesenchymal stem cells through direct targeting of lymphoid enhancer-binding factor-1

Authors
 Seungil Paik  ;  Ho Sun Jung  ;  Jin Woo Lee  ;  Min Sung Park  ;  Dong Suk Yoon  ;  Seulgi Lee 
Citation
 Stem Cells and Development, Vol.21(18) : 3298-3308, 2012 
Journal Title
 Stem Cells and Development 
ISSN
 1547-3287 
Issue Date
2012
Abstract
microRNAs are small molecules, about 17-23 nucleotides in length, that act as translational regulators of their target gene. By binding to a target, microRNAs are known to either inhibit translation or induce degradation of the target. Despite the great interest in microRNAs, however, the exact targets of each individual microRNA in different processes remain largely unknown. In this study, we determined that the lymphoid enhancer-binding factor-1 (LEF-1) was expressed during the chondrogenesis of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and sought to identify a novel microRNA targeting this gene. Through subsequent studies, we have identified, for the first time, one particular microRNA, miR-449a, that recognizes and regulates the expression of LEF-1 in a dose-dependent and sequence-specific manner. In addition, we observed that the inhibition of LEF-1 via miR-449a led to the subsequent repression of Sox 9, which is a well-established regulator of chondrogenesis. Collectively, this study demonstrated that miR-449a directly targets LEF-1, which in turn affects the expression of Sox 9, ultimately leading to the proper regulation of the differentiation and chondrogenesis of human MSCs (hBM-MSCs).
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91873
DOI
10.1089/scd.2011.0732
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
Yonsei Authors
박민성(Park, Min Sung) ; 백승일(Paik, Seung Il) ; 윤동석(Yoon, Dong Suk) ; 이슬기(Lee, Seul Gi) ; 이진우(Lee, Jin Woo) ; 정호선(Jung, Ho Sun)
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Full Text
http://online.liebertpub.com/doi/abs/10.1089/scd.2011.0732
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