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MicroRNA-1280 Inhibits Invasion and Metastasis by Targeting ROCK1 in Bladder Cancer

DC FieldValueLanguage
dc.contributor.author장인익-
dc.date.accessioned2014-12-19T17:37:00Z-
dc.date.available2014-12-19T17:37:00Z-
dc.date.issued2012-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91744-
dc.description.abstractMicroRNAs (miRNAs) are non-protein-coding sequences that can function as oncogenes or tumor suppressor genes. This study documents the tumor suppressor role of miR-1280 in bladder cancer. Quantitative real-time PCR and in situ hybridization analyses showed that miR-1280 is significantly down-regulated in bladder cancer cell lines and tumors compared to a non-malignant cell line or normal tissue samples. To decipher the functional significance of miR-1280 in bladder cancer, we ectopically over-expressed miR-1280 in bladder cancer cell lines. Over-expression of miR-1280 had antiproliferative effects and impaired colony formation of bladder cancer cell lines. FACS (fluorescence activated cell sorting) analysis revealed that re-expression of miR-1280 in bladder cancer cells induced G2-M cell cycle arrest and apoptosis. Our results demonstrate that miR-1280 inhibited migration and invasion of bladder cancer cell lines. miR-1280 also attenuated ROCK1 and RhoC protein expression. Luciferase reporter assays demonstrated that oncogene ROCK1 is a direct target of miR-1280 in bladder cancer. This study also indicates that miR-1280 may be of diagnostic and prognostic importance in bladder cancer. For instance, ROC analysis showed that miR-1280 expression can distinguish between malignant and normal bladder cancer cases and Kaplan-Meier analysis revealed that patients with miR-1280 high expression had higher overall survival compared to those with low miR-1280 expression. In conclusion, this is the first study to document that miR-1280 functions as a tumor suppressor by targeting oncogene ROCK1 to invasion/migration and metastasis. Various compounds are currently being used as ROCK1 inhibitors; therefore restoration of tumor suppressor miR-1280 might be therapeutically useful either alone or in combination with these compounds in the treatment of bladder cancer.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfPLOS ONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHApoptosis/genetics-
dc.subject.MESHApoptosis/physiology-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Survival/genetics-
dc.subject.MESHCell Survival/physiology-
dc.subject.MESHGene Expression Regulation, Neoplastic/genetics-
dc.subject.MESHGene Expression Regulation, Neoplastic/physiology-
dc.subject.MESHHumans-
dc.subject.MESHImmunoblotting-
dc.subject.MESHIn Situ Hybridization-
dc.subject.MESHIn Vitro Techniques-
dc.subject.MESHMicroRNAs/metabolism*-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHUrinary Bladder Neoplasms/genetics*-
dc.subject.MESHrho-Associated Kinases/genetics-
dc.subject.MESHrho-Associated Kinases/metabolism*-
dc.titleMicroRNA-1280 Inhibits Invasion and Metastasis by Targeting ROCK1 in Bladder Cancer-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorShahana Majid-
dc.contributor.googleauthorAltaf A. Dar-
dc.contributor.googleauthorSharanjot Saini-
dc.contributor.googleauthorVarahram Shahryari-
dc.contributor.googleauthorSumit Arora-
dc.contributor.googleauthorMohd Saif Zaman-
dc.contributor.googleauthorInik Chang-
dc.contributor.googleauthorSoichiro Yamamura-
dc.contributor.googleauthorTakeshi Chiyomaru-
dc.contributor.googleauthorShinichiro Fukuhara-
dc.contributor.googleauthorYuichiro Tanaka-
dc.contributor.googleauthorGuoren Deng-
dc.contributor.googleauthorZ. Laura Tabatabai-
dc.contributor.googleauthorRajvir Dahiya-
dc.identifier.doi23056431-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03461-
dc.relation.journalcodeJ02540-
dc.identifier.eissn1932-6203-
dc.identifier.pmid23056431-
dc.subject.keywordApoptosis/genetics-
dc.subject.keywordApoptosis/physiology-
dc.subject.keywordCell Line, Tumor-
dc.subject.keywordCell Survival/genetics-
dc.subject.keywordCell Survival/physiology-
dc.subject.keywordGene Expression Regulation, Neoplastic/genetics-
dc.subject.keywordGene Expression Regulation, Neoplastic/physiology-
dc.subject.keywordHumans-
dc.subject.keywordImmunoblotting-
dc.subject.keywordIn Situ Hybridization-
dc.subject.keywordIn Vitro Techniques-
dc.subject.keywordMicroRNAs/metabolism*-
dc.subject.keywordReal-Time Polymerase Chain Reaction-
dc.subject.keywordUrinary Bladder Neoplasms/genetics*-
dc.subject.keywordrho-Associated Kinases/genetics-
dc.subject.keywordrho-Associated Kinases/metabolism*-
dc.contributor.alternativeNameChang, In Ik-
dc.contributor.affiliatedAuthorChang, In Ik-
dc.citation.volume7-
dc.citation.number10-
dc.citation.startPagee46743-
dc.identifier.bibliographicCitationPLOS ONE, Vol.7(10) : e46743, 2012-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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