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Inhibition of PTEN Gene Expression by Oncogenic miR-23b-3p in Renal Cancer

DC FieldValueLanguage
dc.contributor.author장인익-
dc.date.accessioned2014-12-19T17:36:58Z-
dc.date.available2014-12-19T17:36:58Z-
dc.date.issued2012-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91743-
dc.description.abstractBACKGROUND: miR-23b is located on chromosome number 9 and plays different roles in different organs especially with regards to cancer development. However, the functional significance of miR-23b-3p in renal cell carcinoma (RCC) has not been reported. METHODS AND RESULTS: We measured miR-23b-3p levels in 29 pairs of renal cell carcinoma and their normal matched tissues using real-time PCR. The expression level of miR-23b-3p was correlated with the 5 year survival rate of renal cancer patients. In 15 cases (52%), miR-23b-3p expression was found to be high. All patients with moderate to low miR-23b-3p expression survived 5 years, while those with high miR-23b-3p expression, only 50% survived. After knocking down miRNA-23b-3p expression in RCC cell lines, there was an induction of apoptosis and reduced invasive capabilities. MiR-23b-3p was shown to directly target PTEN gene through 3'UTR reporter assays. Inhibition of miR-23b-3p induces PTEN gene expression with a concomitant reduction in PI3-kinase, total Akt and IL-32. Immunohistochemistry showed the lack of PTEN protein expression in cancerous regions of tissue samples where the expression of miR-23b-3p was high. We studied the in vitro effects of the dietary chemo preventive agent genistein on miR-23b-3p expression and found that it inhibited expression of miR-23b-3p in RCC cell lines. CONCLUSIONS: The current study shows that miR-23b-3p is an oncogenic miRNA and inhibits PTEN tumor suppressor gene in RCC. Therefore, inhibition of miR-23b-3p may be a useful therapeutic target for the treatment of renal cell carcinoma.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfPLOS ONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHApoptosis/genetics-
dc.subject.MESHBase Sequence-
dc.subject.MESHCarcinoma, Renal Cell/genetics*-
dc.subject.MESHCarcinoma, Renal Cell/metabolism-
dc.subject.MESHCarcinoma, Renal Cell/mortality-
dc.subject.MESHCell Cycle/genetics-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Movement/genetics-
dc.subject.MESHGene Expression Regulation, Neoplastic*-
dc.subject.MESHGenistein/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHKidney Neoplasms/genetics*-
dc.subject.MESHKidney Neoplasms/metabolism-
dc.subject.MESHKidney Neoplasms/mortality-
dc.subject.MESHMicroRNAs/genetics*-
dc.subject.MESHMicroRNAs/metabolism-
dc.subject.MESHPTEN Phosphohydrolase/genetics*-
dc.subject.MESHPTEN Phosphohydrolase/metabolism-
dc.subject.MESHRNA Interference-
dc.titleInhibition of PTEN Gene Expression by Oncogenic miR-23b-3p in Renal Cancer-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorMohd Saif Zaman-
dc.contributor.googleauthorSobha Thamminana-
dc.contributor.googleauthorVarahram Shahryari-
dc.contributor.googleauthorTakeshi Chiyomaru-
dc.contributor.googleauthorGuoren Deng-
dc.contributor.googleauthorSharanjot Saini-
dc.contributor.googleauthorShahana Majid-
dc.contributor.googleauthorShinichiro Fukuhara-
dc.contributor.googleauthorInik Chang-
dc.contributor.googleauthorSumit Arora-
dc.contributor.googleauthorHiroshi Hirata-
dc.contributor.googleauthorKoji Ueno-
dc.contributor.googleauthorKamaldeep Singh-
dc.contributor.googleauthorYuichiro Tanaka-
dc.contributor.googleauthorRajvir Dahiya-
dc.identifier.doi23189187-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03461-
dc.relation.journalcodeJ02540-
dc.identifier.eissn1932-6203-
dc.identifier.pmid23189187-
dc.subject.keywordApoptosis/genetics-
dc.subject.keywordBase Sequence-
dc.subject.keywordCarcinoma, Renal Cell/genetics*-
dc.subject.keywordCarcinoma, Renal Cell/metabolism-
dc.subject.keywordCarcinoma, Renal Cell/mortality-
dc.subject.keywordCell Cycle/genetics-
dc.subject.keywordCell Line, Tumor-
dc.subject.keywordCell Movement/genetics-
dc.subject.keywordGene Expression Regulation, Neoplastic*-
dc.subject.keywordGenistein/metabolism-
dc.subject.keywordHumans-
dc.subject.keywordKidney Neoplasms/genetics*-
dc.subject.keywordKidney Neoplasms/metabolism-
dc.subject.keywordKidney Neoplasms/mortality-
dc.subject.keywordMicroRNAs/genetics*-
dc.subject.keywordMicroRNAs/metabolism-
dc.subject.keywordPTEN Phosphohydrolase/genetics*-
dc.subject.keywordPTEN Phosphohydrolase/metabolism-
dc.subject.keywordRNA Interference-
dc.contributor.alternativeNameChang, In Ik-
dc.contributor.affiliatedAuthorChang, In Ik-
dc.citation.volume7-
dc.citation.number11-
dc.citation.startPagee50203-
dc.identifier.bibliographicCitationPLOS ONE, Vol.7(11) : e50203, 2012-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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