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Krüppel-like factor KLF8 plays a critical role in adipocyte differentiation

DC FieldValueLanguage
dc.contributor.author김재우-
dc.contributor.author김효정-
dc.contributor.author이유정-
dc.date.accessioned2014-12-19T17:36:39Z-
dc.date.available2014-12-19T17:36:39Z-
dc.date.issued2012-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91733-
dc.description.abstractKLF8 (Krüppel-like factor 8) is a zinc-finger transcription factor known to play an essential role in the regulation of the cell cycle, apoptosis, and differentiation. However, its physiological roles and functions in adipogenesis remain unclear. In the present study, we show that KLF8 acts as a key regulator controlling adipocyte differentiation. In 3T3-L1 preadipocytes, we found that KLF8 expression was induced during differentiation, which was followed by expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). Adipocyte differentiation was significantly attenuated by the addition of siRNA against KLF8, whereas overexpression of KLF8 resulted in enhanced differentiation. Furthermore, luciferase reporter assays demonstrated that overexpression of KLF8 induced PPARγ2 and C/EBPα promoter activity, suggesting that KLF8 is an upstream regulator of PPARγ and C/EBPα. The KLF8 binding sites were localized by site mutation analysis to -191 region in C/EBPα promoter and -303 region in PPARγ promoter, respectively. Taken together, these data reveal that KLF8 is a key component of the transcription factor network that controls terminal differentiation during adipogenesis.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfPLOS ONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH3T3-L1 Cells-
dc.subject.MESHAdipocytes/cytology*-
dc.subject.MESHAdipocytes/metabolism*-
dc.subject.MESHAdipose Tissue/cytology-
dc.subject.MESHAnimals-
dc.subject.MESHBase Sequence-
dc.subject.MESHCCAAT-Enhancer-Binding Protein-alpha/genetics-
dc.subject.MESHCell Differentiation*/genetics-
dc.subject.MESHClone Cells-
dc.subject.MESHGene Expression Regulation-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHMice-
dc.subject.MESHMitosis-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPPAR gamma/genetics-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHProtein Binding/genetics-
dc.subject.MESHTranscription Factors/genetics-
dc.subject.MESHTranscription Factors/metabolism*-
dc.subject.MESHTranscription, Genetic-
dc.titleKrüppel-like factor KLF8 plays a critical role in adipocyte differentiation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorHaemi Lee-
dc.contributor.googleauthorHyo Jung Kim-
dc.contributor.googleauthorYoo Jeong Lee-
dc.contributor.googleauthorMin-Young Lee-
dc.contributor.googleauthorHyeonjin Choi-
dc.contributor.googleauthorHyemin Lee-
dc.contributor.googleauthorJae-woo Kim-
dc.identifier.doi23285057-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00865-
dc.contributor.localIdA01204-
dc.contributor.localIdA03014-
dc.relation.journalcodeJ02540-
dc.identifier.eissn1932-6203-
dc.identifier.pmid23285057-
dc.subject.keyword3T3-L1 Cells-
dc.subject.keywordAdipocytes/cytology*-
dc.subject.keywordAdipocytes/metabolism*-
dc.subject.keywordAdipose Tissue/cytology-
dc.subject.keywordAnimals-
dc.subject.keywordBase Sequence-
dc.subject.keywordCCAAT-Enhancer-Binding Protein-alpha/genetics-
dc.subject.keywordCell Differentiation*/genetics-
dc.subject.keywordClone Cells-
dc.subject.keywordGene Expression Regulation-
dc.subject.keywordGene Knockdown Techniques-
dc.subject.keywordMice-
dc.subject.keywordMitosis-
dc.subject.keywordMolecular Sequence Data-
dc.subject.keywordPPAR gamma/genetics-
dc.subject.keywordPromoter Regions, Genetic-
dc.subject.keywordProtein Binding/genetics-
dc.subject.keywordTranscription Factors/genetics-
dc.subject.keywordTranscription Factors/metabolism*-
dc.subject.keywordTranscription, Genetic-
dc.contributor.alternativeNameKim, Jae Woo-
dc.contributor.alternativeNameKim, Hyo Jung-
dc.contributor.alternativeNameLee, Yoo Jeong-
dc.contributor.affiliatedAuthorKim, Jae Woo-
dc.contributor.affiliatedAuthorKim, Hyo Jung-
dc.contributor.affiliatedAuthorLee, Yoo Jeong-
dc.citation.volume7-
dc.citation.number12-
dc.citation.startPagee52474-
dc.identifier.bibliographicCitationPLOS ONE, Vol.7(12) : e52474, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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