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Excretory-secretory products of Giardia lamblia induce interleukin-8 production in human colonic cells via activation of p38, ERK1/2, NF-κB and AP-1
DC Field | Value | Language |
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dc.contributor.author | 박순정 | - |
dc.contributor.author | 용태순 | - |
dc.contributor.author | 이혜연 | - |
dc.date.accessioned | 2014-12-19T17:33:37Z | - |
dc.date.available | 2014-12-19T17:33:37Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0141-9838 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91637 | - |
dc.description.abstract | Giardia lamblia, a pathogen causing diarrhoeal outbreaks, is interesting how it triggers immune response in the human epithelial cells. This study defined the crucial roles of signalling components involved in G. lamblia-induced cytokine production in human epithelial cells. Incubation of the gastrointestinal cell line HT-29 with G. lamblia GS trophozoites triggered production of interleukin (IL)-1β, IL-8 and tumour necrosis factor (TNF)-α. IL-8 production was not significantly decreased by physically separating the HT-29 cells and G. lamblia GS trophozoites. Indeed, treatment of HT-29 with G. lamblia excretory-secretory products (ESP) induced IL-8 production. Electrophoretic mobility gel shift and transfection assays using mutagenized IL-8 promoter reporter plasmids indicated that IL-8 production by G. lamblia ESP occurs through activation of two transcriptional factors, nuclear factor kappaB (NF-κB) and activator protein 1 (AP-1) in HT-29 cells. In addition, activation of two mitogen-activated protein kinases (MAPKs), p38 and ERK1/2, was also detected in the HT-29 cells stimulated with G. lamblia ESP. Selective inhibition of these MAPKs resulted in decreased production of ESP-induced IL-8. These results indicate that activation of p38, ERK1/2 MAPK, NF-κB and AP-1 comprises the signalling pathway responsible for IL-8 production by G. lamblia ESP | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 183~198 | - |
dc.relation.isPartOf | PARASITE IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Colon/cytology | - |
dc.subject.MESH | Colon/immunology* | - |
dc.subject.MESH | Colon/parasitology | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Giardia lamblia/genetics | - |
dc.subject.MESH | Giardia lamblia/growth & development | - |
dc.subject.MESH | Giardia lamblia/metabolism | - |
dc.subject.MESH | Giardia lamblia/pathogenicity* | - |
dc.subject.MESH | HT29 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-8/biosynthesis* | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/genetics | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/metabolism | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3/genetics | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3/metabolism | - |
dc.subject.MESH | NF-kappa B/genetics | - |
dc.subject.MESH | NF-kappa B/metabolism | - |
dc.subject.MESH | Protozoan Proteins/genetics | - |
dc.subject.MESH | Protozoan Proteins/immunology* | - |
dc.subject.MESH | Protozoan Proteins/metabolism | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Transcription Factor AP-1/genetics | - |
dc.subject.MESH | Transcription Factor AP-1/metabolism | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/genetics | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/metabolism | - |
dc.title | Excretory-secretory products of Giardia lamblia induce interleukin-8 production in human colonic cells via activation of p38, ERK1/2, NF-κB and AP-1 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | H.-Y. LEE | - |
dc.contributor.googleauthor | S. HYUNG | - |
dc.contributor.googleauthor | N. Y. LEE | - |
dc.contributor.googleauthor | T.-S. YONG | - |
dc.contributor.googleauthor | S.-H. HAN | - |
dc.contributor.googleauthor | S.-J. PARK | - |
dc.identifier.doi | 22224945 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01545 | - |
dc.contributor.localId | A02424 | - |
dc.contributor.localId | A03314 | - |
dc.relation.journalcode | J02466 | - |
dc.identifier.eissn | 1365-3024 | - |
dc.identifier.pmid | 22224945 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3024.2012.01354.x/abstract | - |
dc.subject.keyword | excretory–secretory product | - |
dc.subject.keyword | Giardia lamblia | - |
dc.subject.keyword | HT-29 cells | - |
dc.subject.keyword | mitogen-activated protein kinase | - |
dc.subject.keyword | transcription factor | - |
dc.contributor.alternativeName | Park, Soon Jung | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.alternativeName | Lee, Hye Yeon | - |
dc.contributor.affiliatedAuthor | Park, Soon Jung | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Lee, Hye Yeon | - |
dc.citation.volume | 34 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 183 | - |
dc.citation.endPage | 198 | - |
dc.identifier.bibliographicCitation | PARASITE IMMUNOLOGY, Vol.34(4) : 183-198, 2012 | - |
dc.identifier.rimsid | 29525 | - |
dc.type.rims | ART | - |
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