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Regulation of Wnt/β-catenin signaling by CCAAT/enhancer binding protein β during adipogenesis
DC Field | Value | Language |
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dc.contributor.author | 김재우 | - |
dc.date.accessioned | 2014-12-19T17:31:45Z | - |
dc.date.available | 2014-12-19T17:31:45Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1930-7381 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91579 | - |
dc.description.abstract | Activation of the Wnt/β-catenin signaling pathway inhibits adipogenesis, while disruption of Wnt signaling leads to spontaneous adipogenesis. CCAAT/enhancer binding protein β (C/EBPβ) is rapidly induced in early stages of adipogenesis and is responsible for transcriptional induction of two major adipogenic transcription factors, peroxisome proliferator-activated receptor γ (PPARγ) and C/EBPα. In this study, we examined whether C/EBPβ is involved in the suppression of Wnt/β-catenin signaling during adipogenesis. Knockdown of C/EBPβ expression not only inhibited adipogenesis but also maintained active Wnt/β-catenin signaling, after addition of adipogenic inducers. In contrast, overexpression of C/EBPβ substantially inhibited Wnt signaling. Interestingly, our data showed that C/EBPβ is involved in the expression of Wnt10b, a major Wnt ligand in preadipocytes, even though C/EBPβ is not an essential factor to regulate Wnt10b expression during adipogenesis, and that C/EBPβ inhibits Wnt10b promoter activity by directly binding to specific regions of the promoter. These results suggest a dual function of C/EBPβ: stimulating expression of adipogenic genes and inhibiting Wnt signaling. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | OBESITY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipocytes/metabolism* | - |
dc.subject.MESH | Adipogenesis*/genetics | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Protein-beta/genetics | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Protein-beta/metabolism* | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | PPAR gamma/genetics | - |
dc.subject.MESH | PPAR gamma/metabolism* | - |
dc.subject.MESH | Proto-Oncogene Proteins/genetics | - |
dc.subject.MESH | Proto-Oncogene Proteins/metabolism* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Wnt Proteins/genetics | - |
dc.subject.MESH | Wnt Proteins/metabolism* | - |
dc.subject.MESH | Wnt Signaling Pathway*/genetics | - |
dc.title | Regulation of Wnt/β-catenin signaling by CCAAT/enhancer binding protein β during adipogenesis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Sung Soo Chung | - |
dc.contributor.googleauthor | Ji Seon Lee | - |
dc.contributor.googleauthor | Min Kim | - |
dc.contributor.googleauthor | Byung Yong Ahn | - |
dc.contributor.googleauthor | Hye Seung Jung | - |
dc.contributor.googleauthor | Hak Mo Lee | - |
dc.contributor.googleauthor | Jae-Woo Kim | - |
dc.contributor.googleauthor | Kyong Soo Park | - |
dc.identifier.doi | 21760632 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00865 | - |
dc.relation.journalcode | J02404 | - |
dc.identifier.eissn | 1930-739X | - |
dc.identifier.pmid | 21760632 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1038/oby.2011.212/abstract | - |
dc.subject.keyword | Adipocytes/metabolism* | - |
dc.subject.keyword | Adipogenesis*/genetics | - |
dc.subject.keyword | Blotting, Western | - |
dc.subject.keyword | CCAAT-Enhancer-Binding Protein-beta/genetics | - |
dc.subject.keyword | CCAAT-Enhancer-Binding Protein-beta/metabolism* | - |
dc.subject.keyword | Cell Differentiation | - |
dc.subject.keyword | Cells, Cultured | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | PPAR gamma/genetics | - |
dc.subject.keyword | PPAR gamma/metabolism* | - |
dc.subject.keyword | Proto-Oncogene Proteins/genetics | - |
dc.subject.keyword | Proto-Oncogene Proteins/metabolism* | - |
dc.subject.keyword | Real-Time Polymerase Chain Reaction | - |
dc.subject.keyword | Signal Transduction | - |
dc.subject.keyword | Wnt Proteins/genetics | - |
dc.subject.keyword | Wnt Proteins/metabolism* | - |
dc.subject.keyword | Wnt Signaling Pathway*/genetics | - |
dc.contributor.alternativeName | Kim, Jae Woo | - |
dc.contributor.affiliatedAuthor | Kim, Jae Woo | - |
dc.citation.volume | 20 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 482 | - |
dc.citation.endPage | 487 | - |
dc.identifier.bibliographicCitation | OBESITY, Vol.20(3) : 482-487, 2012 | - |
dc.identifier.rimsid | 29317 | - |
dc.type.rims | ART | - |
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