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LIME mediates immunological synapse formation through activation of VAV

Authors
 Myoungsun Son  ;  Inyoung Park  ;  Ok-Hee Lee  ;  Inmoo Rhee  ;  Changwon Park  ;  Yungdae Yun 
Citation
 MOLECULES AND CELLS, Vol.33(4) : 407-414, 2012 
Journal Title
MOLECULES AND CELLS
ISSN
 1016-8478 
Issue Date
2012
MeSH
Actins*/metabolism ; Adaptor Proteins, Signal Transducing/metabolism ; Adaptor Proteins, Vesicular Transport*/genetics ; Adaptor Proteins, Vesicular Transport*/immunology ; Adaptor Proteins, Vesicular Transport*/metabolism ; Binding Sites ; Cell Adhesion*/genetics ; Cell Adhesion*/immunology ; Gene Expression Regulation, Developmental*/immunology ; Humans ; Immunological Synapses*/metabolism ; Integrins/metabolism ; Intracellular Signaling Peptides and Proteins/metabolism ; Jurkat Cells ; Membrane Proteins/metabolism ; Mitochondrial Proteins/metabolism ; Protein Binding ; Proto-Oncogene Proteins c-vav*/genetics ; Proto-Oncogene Proteins c-vav*/immunology ; Proto-Oncogene Proteins c-vav*/metabolism ; Receptors, Antigen, T-Cell/metabolism ; Signal Transduction/immunology ; Transcriptional Activation ; rac1 GTP-Binding Protein/metabolism
Keywords
adhesion ; immunological synapse ; integrin ; LIME ; VAV
Abstract
Lck Interacting Membrane protein (LIME) was previously characterized as a transmembrane adaptor protein mediating TCR-dependent T cell activation. Here, we show that LIME associates with Vav in response to TCR stimulation and is required for Vav guanine nucleotide exchange factor (GEF) activity for Rac1. Consistent with this finding, actin polymerization at the immunological synapse (IS) was markedly enhanced by overexpression of LIME, but was reduced by expression of a LIME shRNA. Moreover, TCR-mediated cell adhesion to ICAM-1, laminin, or fibronectin was downregulated by expression of LIME shRNA. In addition, in the IS, LIME but not LAT was found to localize at the peripheral-supramolecular activation cluster (p-SMAC) where the integrins were previously shown to be localized. Together, these results establish LIME as a transmembrane adaptor protein linking TCR stimulation to IS formation and integrin activation through activation of Vav.
Full Text
http://link.springer.com/article/10.1007%2Fs10059-012-0011-8
DOI
22395814
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
Yonsei Authors
Lee, Ok Hee(이옥희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91482
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