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Differential gel-based proteomic approach for cancer biomarker discovery using human plasma.
DC Field | Value | Language |
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dc.contributor.author | 김호근 | - |
dc.date.accessioned | 2014-12-19T17:27:50Z | - |
dc.date.available | 2014-12-19T17:27:50Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91455 | - |
dc.description.abstract | Two-dimensional fluorescence difference gel electrophoresis (2D DIGE) has become a general platform for analysis of various clinical samples such as biofluids and tissues. In comparison to conventional 2-D polyacrylamide gel electrophoresis (2D PAGE), 2D DIGE offers several advantages, such as accuracy and reproducibility between experiments, which facilitate spot-to-spot comparisons. Although whole plasma can be easily obtained, the complexity of plasma samples makes it challenging to analyze samples with good reproducibility. Here, we describe a method for decreasing protein complexity in plasma samples within a narrow pH range by depleting high-abundance plasma proteins. In combination with analysis of differentially expressed spots, trypsin digestion, identification of protein by mass spectrometry, and standard 2D PAGE and DIGE, this method has been optimized for comparison of plasma samples from healthy donors and patients diagnosed with hepatocellular carcinoma. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | Methods in Molecular Biology (Clifton, N.J.) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Biomarkers, Tumor/blood* | - |
dc.subject.MESH | Biomarkers, Tumor/isolation & purification* | - |
dc.subject.MESH | Biomarkers, Tumor/metabolism | - |
dc.subject.MESH | Carbocyanines/chemistry | - |
dc.subject.MESH | Carcinoma, Hepatocellular/blood | - |
dc.subject.MESH | Chromatography, Liquid | - |
dc.subject.MESH | Coloring Agents/chemistry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hydrogen-Ion Concentration | - |
dc.subject.MESH | Image Processing, Computer-Assisted | - |
dc.subject.MESH | Liver Neoplasms/blood | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Nanotechnology | - |
dc.subject.MESH | Proteolysis | - |
dc.subject.MESH | Proteome/analysis* | - |
dc.subject.MESH | Proteome/isolation & purification* | - |
dc.subject.MESH | Proteome/metabolism | - |
dc.subject.MESH | Proteomics/methods* | - |
dc.subject.MESH | Staining and Labeling | - |
dc.subject.MESH | Tandem Mass Spectrometry | - |
dc.subject.MESH | Trypsin/metabolism | - |
dc.subject.MESH | Two-Dimensional Difference Gel Electrophoresis/methods* | - |
dc.title | Differential gel-based proteomic approach for cancer biomarker discovery using human plasma. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Keun Na | - |
dc.contributor.googleauthor | Min-Jung Lee | - |
dc.contributor.googleauthor | Hye-Jin Jeong | - |
dc.contributor.googleauthor | Hoguen Kim | - |
dc.contributor.googleauthor | Young-Ki Paik | - |
dc.identifier.doi | 22311764 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01183 | - |
dc.relation.journalcode | J02226 | - |
dc.identifier.pmid | 22311764 | - |
dc.identifier.url | http://link.springer.com/protocol/10.1007%2F978-1-61779-573-2_16 | - |
dc.subject.keyword | Two-dimensional fluorescence difference gel electrophoresis | - |
dc.subject.keyword | Narrow pH range | - |
dc.subject.keyword | Plasma proteomics | - |
dc.subject.keyword | Hepatocellular carcinoma | - |
dc.subject.keyword | Biomarker | - |
dc.contributor.alternativeName | Kim, Ho Keun | - |
dc.contributor.affiliatedAuthor | Kim, Ho Keun | - |
dc.citation.volume | 854 | - |
dc.citation.startPage | 223 | - |
dc.citation.endPage | 237 | - |
dc.identifier.bibliographicCitation | Methods in Molecular Biology (Clifton, N.J.), Vol.854 : 223-237, 2012 | - |
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