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Hyperglycemia attenuates myocardial preconditioning of remifentanil

DC Field Value Language
dc.contributor.author곽영란-
dc.contributor.author김소연-
dc.contributor.author심연희-
dc.contributor.author황기철-
dc.date.accessioned2014-12-19T17:16:38Z-
dc.date.available2014-12-19T17:16:38Z-
dc.date.issued2012-
dc.identifier.issn0022-4804-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91109-
dc.description.abstractBACKGROUND: Hyperglycemia attenuates cardioprotection by remifentanil-preconditioning in ischemia-reperfusion in vivo in diabetic rats. However, the effects of hyperglycemia in cultured ventricular myocytes remains unknown. Therefore, we examined the in vitro effects of hyperglycemia on hypoxia-reoxygenation (H/R) and cardioprotection from remifentanil-preconditioning in isolated neonatal rat ventricular myocytes (NRVMs), including effects on apoptotic signaling pathways and Ca(2+) homeostasis. MATERIALS AND METHODS: NRVMs were cultured in medium with 5.5 mM (normoglycemia) or 25.5 mM glucose for one day. Then, NRVMs in H/R groups were exposed to 1 h of hypoxia and 5 h of reoxygenation with or without remifentanil-preconditioning at 1 μM. Cell viability, apoptosis, and Ca(2+) homeostasis were assessed by MTT assay, caspase-3 assay, confocal microscopy and immunoblots. RESULTS: In normoglycemia, remifentanil-preconditioning improved the viability of cardiomyocytes (P < 0.01) and prevented the increase of caspase-3 activity and Ca(2+) overload after H/R injury (P < 0.05). In addition, decrease in Akt, ERK1/2, and Bcl-2, and the increase in Bax by H/R was attenuated by remifentanil-preconditioning (P < 0.05). However, in hyperglycemia, the viability was partially impaired after H/R but not improved by remifentanil-preconditioning. Apoptotic activity, Ca(2+) concentration, and apoptotic kinases except Akt were not affected by either H/R or remifentanil-preconditioning under hyperglycemia. Akt phosphorylation was decreased by H/R but not restored by remifentanil preconditioning. CONCLUSIONS: Remifentanil preconditioning under normoglycemia renders NRVMs resistant to H/R injury by reducing apoptosis and intracellular Ca(2+) concentrations. The mechanism appears to be modulation of apoptotic signaling. However, hyperglycemia mitigates H/R injury in NRVMs, and may reduce the protective effect of remifentanil-preconditioning that may be associated with the Akt pathways.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF SURGICAL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnalgesics, Opioid/pharmacology-
dc.subject.MESHAnalgesics, Opioid/therapeutic use*-
dc.subject.MESHAnimals-
dc.subject.MESHAnimals, Newborn-
dc.subject.MESHApoptosis/drug effects-
dc.subject.MESHCalcium/metabolism-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHCells, Cultured-
dc.subject.MESHExtracellular Signal-Regulated MAP Kinases/metabolism-
dc.subject.MESHHyperglycemia/complications*-
dc.subject.MESHHyperglycemia/metabolism-
dc.subject.MESHHypoxia/complications*-
dc.subject.MESHHypoxia/drug therapy-
dc.subject.MESHHypoxia/metabolism-
dc.subject.MESHIschemic Preconditioning, Myocardial/methods*-
dc.subject.MESHMyocytes, Cardiac/drug effects-
dc.subject.MESHMyocytes, Cardiac/metabolism-
dc.subject.MESHPiperidines/pharmacology-
dc.subject.MESHPiperidines/therapeutic use*-
dc.subject.MESHProto-Oncogene Proteins c-akt/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.titleHyperglycemia attenuates myocardial preconditioning of remifentanil-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Anesthesiology (마취통증의학)-
dc.contributor.googleauthorHyun Soo Kim-
dc.contributor.googleauthorSo Yeon Kim-
dc.contributor.googleauthorYoung Lan Kwak-
dc.contributor.googleauthorKi Chul Hwang-
dc.contributor.googleauthorYon Hee Shim-
dc.identifier.doi21392805-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00172-
dc.contributor.localIdA02196-
dc.contributor.localIdA04456-
dc.contributor.localIdA00616-
dc.relation.journalcodeJ01763-
dc.identifier.eissn1095-8673-
dc.identifier.pmid21392805-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0022480411000552-
dc.subject.keywordhyperglycemia-
dc.subject.keywordhypoxia-reoxygenation injury-
dc.subject.keywordremifentanil-
dc.subject.keywordmyocardial preconditioning-
dc.contributor.alternativeNameKwak, Young Lan-
dc.contributor.alternativeNameKim, So Yeon-
dc.contributor.alternativeNameShim, Yon Hee-
dc.contributor.alternativeNameHwang, Ki Chul-
dc.contributor.affiliatedAuthorKwak, Young Lan-
dc.contributor.affiliatedAuthorShim, Yon Hee-
dc.contributor.affiliatedAuthorHwang, Ki Chul-
dc.contributor.affiliatedAuthorKim, So Yeon-
dc.citation.volume174-
dc.citation.number2-
dc.citation.startPage231-
dc.citation.endPage237-
dc.identifier.bibliographicCitationJOURNAL OF SURGICAL RESEARCH, Vol.174(2) : 231-237, 2012-
dc.identifier.rimsid33923-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers

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