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Kr-pok increases FASN expression by modulating the DNA binding of SREBP-1c and Sp1 at the proximal promoter

DC FieldValueLanguage
dc.contributor.author고동인-
dc.contributor.author김민경-
dc.contributor.author윤재현-
dc.contributor.author전부남-
dc.contributor.author최원일-
dc.contributor.author허만욱-
dc.date.accessioned2014-12-19T17:11:18Z-
dc.date.available2014-12-19T17:11:18Z-
dc.date.issued2012-
dc.identifier.issn0022-2275-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90939-
dc.description.abstractKr-pok (kidney cancer-related POZ domain and Krüppel-like protein) is a new proto-oncogenic POZ-domain transcription factor. Fatty acid synthase gene (FASN) encodes one of the key enzymes in fatty acids synthesis and is the only enzyme that synthesizes fatty acids in cancer cells. Sp1 and SREBP-1c are the two major transcription activators of FASN. We investigated whether Kr-pok modulates transcription of the FASN. FASN expression is significantly decreased in Kr-pok knockout murine embryonic fibroblasts. Coimmunoprecipitation, GST fusion protein pull-down, and immunocytochemistry assays show that the zinc-finger domain of Kr-pok interacts directly with the bZIP DNA binding domain of SREBP-1. Electrophoretic mobility shift assay, oligonucleotide pull-down, and chromatin immunoprecipitation assays showed that Kr-pok changes the transcription factor binding dynamics of Sp1 and SREBP-1c to the SRE/E-box elements of the proximal promoter. We found that Kr-pok expression increased during 3T3-L1 preadipocyte differentiation and that FASN expression is decreased by the knockdown of Kr-pok. Kr-pok facilitates the SREBP-1c-mediated preadipocyte differentiation and/or fatty acid synthesis. Kr-pok may act as an important regulator of fatty acid synthesis and may induce rapid cancer cell proliferation by increasing palmitate synthesis.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF LIPID RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH3T3-L1 Cells-
dc.subject.MESHAnimals-
dc.subject.MESHCell Dedifferentiation-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Proliferation-
dc.subject.MESHDoxycycline/pharmacology-
dc.subject.MESHElectrophoretic Mobility Shift Assay-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHFatty Acid Synthase, Type I/genetics-
dc.subject.MESHFatty Acid Synthase, Type I/metabolism*-
dc.subject.MESHFemale-
dc.subject.MESHGene Expression Regulation, Neoplastic-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHHCT116 Cells-
dc.subject.MESHHEK293 Cells-
dc.subject.MESHHumans-
dc.subject.MESHImmunoprecipitation-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHPregnancy-
dc.subject.MESHPromoter Regions, Genetic*-
dc.subject.MESHProtein Binding-
dc.subject.MESHProtein Interaction Domains and Motifs-
dc.subject.MESHProteins/genetics-
dc.subject.MESHProteins/metabolism*-
dc.subject.MESHRecombinant Fusion Proteins/genetics-
dc.subject.MESHRecombinant Fusion Proteins/metabolism-
dc.subject.MESHSterol Regulatory Element Binding Protein 1/genetics-
dc.subject.MESHSterol Regulatory Element Binding Protein 1/metabolism*-
dc.subject.MESHTranscriptional Activation-
dc.titleKr-pok increases FASN expression by modulating the DNA binding of SREBP-1c and Sp1 at the proximal promoter-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorBu-Nam Jeon-
dc.contributor.googleauthorYeon-Sook Kim-
dc.contributor.googleauthorWon-Il Choi-
dc.contributor.googleauthorDong-In Koh-
dc.contributor.googleauthorMin-Kyeong Kim-
dc.contributor.googleauthorJae-Hyeon Yoon-
dc.contributor.googleauthorMin-Young Kim-
dc.contributor.googleauthorBenjamin Hur-
dc.contributor.googleauthorPhilip Dong-Hyun Paik-
dc.contributor.googleauthorMan-Wook Hur-
dc.identifier.doi22331133-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00114-
dc.contributor.localIdA00456-
dc.contributor.localIdA02592-
dc.contributor.localIdA03517-
dc.contributor.localIdA04126-
dc.contributor.localIdA04350-
dc.relation.journalcodeJ01563-
dc.identifier.eissn1539-7262-
dc.identifier.pmid22331133-
dc.subject.keywordkidney cancer-related POZ domain and Krüppel-like protein-
dc.subject.keywordsterol responsive element binding protein-1c-
dc.subject.keywordspecificity protein 1-
dc.subject.keywordfatty acid synthase-
dc.subject.keywordtranscription-
dc.subject.keywordoncoprotein-
dc.subject.keywordlipid-
dc.subject.keywordfatty acid-
dc.contributor.alternativeNameKoh, Dong In-
dc.contributor.alternativeNameKim, Min Kyeong-
dc.contributor.alternativeNameYoon, Jae Hyeon-
dc.contributor.alternativeNameJeon, Bu Nam-
dc.contributor.alternativeNameChoi, Won Il-
dc.contributor.alternativeNameHur, Man Wook-
dc.contributor.affiliatedAuthorKoh, Dong In-
dc.contributor.affiliatedAuthorKim, Min Kyeong-
dc.contributor.affiliatedAuthorYoon, Jae Hyeon-
dc.contributor.affiliatedAuthorJeon, Bu Nam-
dc.contributor.affiliatedAuthorChoi, Won Il-
dc.contributor.affiliatedAuthorHur, Man Wook-
dc.citation.volume53-
dc.citation.number4-
dc.citation.startPage755-
dc.citation.endPage766-
dc.identifier.bibliographicCitationJOURNAL OF LIPID RESEARCH, Vol.53(4) : 755-766, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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