Cited 25 times in
Transcriptional activation of the IL31 gene by NFAT and STAT6
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 송민지 | - |
dc.contributor.author | 이종주 | - |
dc.contributor.author | 김형표 | - |
dc.contributor.author | 박근희 | - |
dc.contributor.author | 박주홍 | - |
dc.date.accessioned | 2014-12-19T17:11:16Z | - |
dc.date.available | 2014-12-19T17:11:16Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0741-5400 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90938 | - |
dc.description.abstract | IL-31, a newly identified member of the IL-6 cytokine family, is involved in many pathological conditions, including atopic dermatitis and pruritis. In this study, we investigated how expression of IL-31 is regulated in T cells and mast cells. We observed that expression of IL-31 required a calcium signal and was dependent on the calcineurin-NFAT signaling pathway. Moreover, we found that IL-31 promoter contains a positive regulatory region that mediates calcium- and IL-4-dependent induction of the IL-31 gene and demonstrated that a change into an open chromatin conformation occurs in this region after stimulation with calcium and IL-4. Whereas IL-4 responsiveness required STAT6 binding sites, calcium responsiveness of IL-31 promoter required NFAT binding sites that bind NFATc1 and NFATc2 in vitro and in vivo. The induction of IL-31 promoter activity was impaired when these sites were mutated but was enhanced by CA-NFATc1 or STAT6 proteins and further increased synergistically by combinations of both proteins. Furthermore, the importance of STAT6 proteins was indicated by impaired, IL-4-mediated induction of IL-31 in STAT6-diminished Jurkat cells. Thus, our data demonstrate that calcium and IL-4 signals are required to mediate induction of IL-31 in Th2 cells and mast cells and that this induction appears to result from specific binding of NFAT and STAT6 proteins. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF LEUKOCYTE BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | CD4-Positive T-Lymphocytes/drug effects | - |
dc.subject.MESH | CD4-Positive T-Lymphocytes/metabolism | - |
dc.subject.MESH | Calcium/physiology | - |
dc.subject.MESH | Dinitrophenols/pharmacology | - |
dc.subject.MESH | HEK293 Cells/drug effects | - |
dc.subject.MESH | HEK293 Cells/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-4/pharmacology | - |
dc.subject.MESH | Interleukins/biosynthesis | - |
dc.subject.MESH | Interleukins/genetics* | - |
dc.subject.MESH | Ionomycin/pharmacology | - |
dc.subject.MESH | Jurkat Cells/drug effects | - |
dc.subject.MESH | Jurkat Cells/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mast Cells/drug effects | - |
dc.subject.MESH | Mast Cells/metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | NFATC Transcription Factors/physiology* | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Recombinant Fusion Proteins/biosynthesis | - |
dc.subject.MESH | Recombinant Fusion Proteins/genetics | - |
dc.subject.MESH | Regulatory Sequences, Nucleic Acid | - |
dc.subject.MESH | STAT6 Transcription Factor/physiology* | - |
dc.subject.MESH | Sequence Alignment | - |
dc.subject.MESH | Sequence Homology, Nucleic Acid | - |
dc.subject.MESH | Serum Albumin/pharmacology | - |
dc.subject.MESH | Tetradecanoylphorbol Acetate/pharmacology | - |
dc.subject.MESH | Transcription, Genetic | - |
dc.title | Transcriptional activation of the IL31 gene by NFAT and STAT6 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Keunhee Park | - |
dc.contributor.googleauthor | Joo-Hong Park | - |
dc.contributor.googleauthor | Wook-Jin Yang | - |
dc.contributor.googleauthor | Jong-Joo Lee | - |
dc.contributor.googleauthor | Min-Ji Song | - |
dc.contributor.googleauthor | Hyoung-Pyo Kim | - |
dc.identifier.doi | 10.1189/jlb.0111020 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02025 | - |
dc.contributor.localId | A03148 | - |
dc.contributor.localId | A01163 | - |
dc.contributor.localId | A01444 | - |
dc.contributor.localId | A01669 | - |
dc.relation.journalcode | J01559 | - |
dc.identifier.eissn | 1938-3673 | - |
dc.identifier.pmid | 22045870 | - |
dc.identifier.url | https://academic.oup.com/jleukbio/article/91/2/245/6960305 | - |
dc.subject.keyword | Th2 | - |
dc.subject.keyword | mast cells | - |
dc.subject.keyword | calcium | - |
dc.subject.keyword | IL-4 | - |
dc.contributor.alternativeName | Song, Min Ji | - |
dc.contributor.alternativeName | Lee, Jong Joo | - |
dc.contributor.alternativeName | Kim, Hyoung Pyo | - |
dc.contributor.alternativeName | Park, Keun Hee | - |
dc.contributor.alternativeName | Park, Joo Hong | - |
dc.contributor.affiliatedAuthor | Song, Min Ji | - |
dc.contributor.affiliatedAuthor | Lee, Jong Joo | - |
dc.contributor.affiliatedAuthor | Kim, Hyoung Pyo | - |
dc.contributor.affiliatedAuthor | Park, Keun Hee | - |
dc.contributor.affiliatedAuthor | Park, Joo Hong | - |
dc.citation.volume | 91 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 245 | - |
dc.citation.endPage | 257 | - |
dc.identifier.bibliographicCitation | JOURNAL OF LEUKOCYTE BIOLOGY, Vol.91(2) : 245-257, 2012 | - |
dc.identifier.rimsid | 33230 | - |
dc.type.rims | ART | - |
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