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N-3 polyunsaturated fatty acid attenuates cholesterol gallstones by suppressing mucin production with a high cholesterol diet in mice

DC FieldValueLanguage
dc.contributor.author김자경-
dc.contributor.author백용한-
dc.contributor.author이동기-
dc.date.accessioned2014-12-19T17:07:31Z-
dc.date.available2014-12-19T17:07:31Z-
dc.date.issued2012-
dc.identifier.issn0815-9319-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90823-
dc.description.abstractBACKGROUND AND AIM: The increasing prevalence of cholesterol gallstone (CG) disease has become an economic burden to the healthcare system. Ursodeoxycholic acid (UDCA) is the only established medical agent used to dissolve gallstones. In investigating novel therapeutics for CG, we assessed the preventive effects of n-3 polyunsaturated fatty acids (n-3PUFA) on the formation of CG induced by feeding a lithogenic diet (LD) containing high cholesterol levels to mice. METHODS: Mice were divided into the following six groups: (A) regular diet (RD); (B) RD+n-3PUFA; (C) LD; (D) LD+n-3PUFA; (E) LD+UDCA; (F) LD+n-3PUFA+UDCA. After RD/LD feeding for 2 weeks, n-3PUFA or UDCA was administered orally and the diet maintained for 8 weeks. The levels of phospholipids and cholesterol in bile, CG formation, gallbladder wall thickness, MUC gene expression in gallbladder were analyzed. RESULTS: No stone or sludge was evident in the RD groups (Groups A, B). Mice in the n-3PUFA treatment (Groups D, F) showed significantly lower stone formation than the other LD groups (Groups C, E). The combination treatment of n-3PUFA and UDCA suppressed stone formation more than mono-therapy with n-3PUFA or UDCA. Bile phospholipid levels were significantly elevated in the Group F. Hypertrophy of the gallbladder wall was evident in mice fed LD. MUC 2, 5AC, 5B and 6 mRNA expression levels were significantly elevated in the LD-fed group, and this was suppressed by n-3PUFA with or without UDCA. CONCLUSIONS: N-3PUFA attenuated gallstone formation in mouse, through increasing the levels of bile phospholipids and suppressing bile mucin formation.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBile/metabolism-
dc.subject.MESHCholagogues and Choleretics/pharmacology-
dc.subject.MESHCholagogues and Choleretics/therapeutic use-
dc.subject.MESHCholesterol/metabolism-
dc.subject.MESHCholesterol, Dietary/pharmacology-
dc.subject.MESHCholesterol, Dietary/therapeutic use*-
dc.subject.MESHDrug Therapy, Combination-
dc.subject.MESHFatty Acids, Omega-3/pharmacology-
dc.subject.MESHFatty Acids, Omega-3/therapeutic use*-
dc.subject.MESHGallbladder/pathology-
dc.subject.MESHGallstones/prevention & control*-
dc.subject.MESHGene Expression/drug effects-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMucin 5AC/genetics-
dc.subject.MESHMucin-2/genetics-
dc.subject.MESHMucin-5B/genetics-
dc.subject.MESHMucin-6/genetics-
dc.subject.MESHMucins/biosynthesis*-
dc.subject.MESHMucins/drug effects-
dc.subject.MESHMucins/genetics-
dc.subject.MESHMucous Membrane/pathology-
dc.subject.MESHPhospholipids/metabolism-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHStatistics, Nonparametric-
dc.subject.MESHUrsodeoxycholic Acid/pharmacology-
dc.subject.MESHUrsodeoxycholic Acid/therapeutic use-
dc.titleN-3 polyunsaturated fatty acid attenuates cholesterol gallstones by suppressing mucin production with a high cholesterol diet in mice-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorJa Kyung Kim-
dc.contributor.googleauthorSoo Min Cho-
dc.contributor.googleauthorSo Hee Kang-
dc.contributor.googleauthorEunjung Kim-
dc.contributor.googleauthorHee Yi-
dc.contributor.googleauthorEun Sun Yun-
dc.contributor.googleauthorDong Goo Lee-
dc.contributor.googleauthorHee Jung Cho-
dc.contributor.googleauthorYong Han Paik-
dc.contributor.googleauthorYang Kyu Choi-
dc.contributor.googleauthorSeung Joo Haam-
dc.contributor.googleauthorHo Chul Shin-
dc.contributor.googleauthorDong Ki Lee-
dc.identifier.doi22849613-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00852-
dc.contributor.localIdA01829-
dc.contributor.localIdA02723-
dc.relation.journalcodeJ01417-
dc.identifier.eissn1440-1746-
dc.identifier.pmid22849613-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1440-1746.2012.07227.x/abstract-
dc.subject.keywordcholesterol gallstone-
dc.subject.keywordhigh cholesterol diet-
dc.subject.keywordmucin-
dc.subject.keywordphopholipid-
dc.subject.keywordn-3 polyunsaturated fatty acid-
dc.contributor.alternativeNameKim, Ja Kyung-
dc.contributor.alternativeNamePaik, Yong Han-
dc.contributor.alternativeNameLee, Dong Ki-
dc.contributor.affiliatedAuthorKim, Ja Kyung-
dc.contributor.affiliatedAuthorPaik, Yong Han-
dc.contributor.affiliatedAuthorLee, Dong Ki-
dc.citation.volume27-
dc.citation.number11-
dc.citation.startPage1745-
dc.citation.endPage1751-
dc.identifier.bibliographicCitationJOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, Vol.27(11) : 1745-1751, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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