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Evaluation of double-disk potentiation and disk potentiation tests using dipicolinic acid for detection of metallo-β-lactamase-producing pseudomonas spp. and Acinetobacter spp

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dc.contributor.author이경원-
dc.contributor.author이양순-
dc.contributor.author정석훈-
dc.contributor.author정윤섭-
dc.contributor.author용동은-
dc.date.accessioned2014-12-19T17:03:23Z-
dc.date.available2014-12-19T17:03:23Z-
dc.date.issued2012-
dc.identifier.issn0095-1137-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90694-
dc.description.abstractAccurate detection of metallo-β-lactamase (MBL)-producing Pseudomonas spp. and Acinetobacter spp. became very important with the increasing prevalence of carbapenem-nonsusceptible clinical isolates. The performance of phenotypic MBL detection methods may depend on the types of MBL and the characteristics of the isolates. A high false-positive rate is a problem with EDTA-based MBL detection methods. We evaluated the performance of double-disk potentiation tests (DDPTs) and disk potentiation tests (DPTs) with dipicolinic acid (DPA) using 44 isolates of Pseudomonas spp. and Acinetobacter spp. producing IMP-1-like, VIM-2-like, and SIM-1 type MBLs. Also, we characterized P. aeruginosa isolates with positive imipenem (IPM)-DPA DDPT, but negative meropenem (MEM)-DPA DDPT, and determined possibility of improving a DDPT by using MacConkey agar. Among five different DDPT methods, the IPM-DPA 250-μg method showed the highest sensitivity (97.7%) and specificity (100%). Among four DPT tests, the highest sensitivity (100%) was shown by the IPM-EDTA 1,900-μg disk method, but the specificity was very low (11.4%). Five of six P. aeruginosa isolates with false-negative DDPTs with MEM-DPA 250-μg disks carried bla(IMP-6,) and the high level resistance to MEM (MIC ≥ 512 μg/ml) was reduced by the presence of phenylalanine arginine β-naphtylamide. Improvement of DDPTs was observed when MacConkey agar was used instead of Mueller-Hinton agar. In conclusion, DPA is a better MBL inhibitor than EDTA for detection of Pseudomonas spp. and Acinetobacter spp. with IMP-1-like, VIM-2-like, and SIM-1-type MBLs. In DPA DDPTs, IPM disks perform better than MEM disks when the isolates are highly resistant to MEM due to the overexpression of efflux pumps.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF CLINICAL MICROBIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcinetobacter/enzymology*-
dc.subject.MESHAcinetobacter/isolation & purification-
dc.subject.MESHAcinetobacter Infections/microbiology-
dc.subject.MESHBacteriological Techniques/methods*-
dc.subject.MESHCulture Media/chemistry-
dc.subject.MESHHumans-
dc.subject.MESHPicolinic Acids/metabolism-
dc.subject.MESHPseudomonas/enzymology*-
dc.subject.MESHPseudomonas/isolation & purification-
dc.subject.MESHPseudomonas Infections/microbiology-
dc.subject.MESHSensitivity and Specificity-
dc.subject.MESHbeta-Lactamases/analysis*-
dc.titleEvaluation of double-disk potentiation and disk potentiation tests using dipicolinic acid for detection of metallo-β-lactamase-producing pseudomonas spp. and Acinetobacter spp-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorYangsoon Lee-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorYunsop Chong-
dc.identifier.doi22837321-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02649-
dc.contributor.localIdA02947-
dc.contributor.localIdA03619-
dc.contributor.localIdA03679-
dc.contributor.localIdA02423-
dc.relation.journalcodeJ01325-
dc.identifier.eissn1098-660X-
dc.identifier.pmid22837321-
dc.subject.keywordAcinetobacter/enzymology*-
dc.subject.keywordAcinetobacter/isolation & purification-
dc.subject.keywordAcinetobacter Infections/microbiology-
dc.subject.keywordBacteriological Techniques/methods*-
dc.subject.keywordCulture Media/chemistry-
dc.subject.keywordHumans-
dc.subject.keywordPicolinic Acids/metabolism-
dc.subject.keywordPseudomonas/enzymology*-
dc.subject.keywordPseudomonas/isolation & purification-
dc.subject.keywordPseudomonas Infections/microbiology-
dc.subject.keywordSensitivity and Specificity-
dc.subject.keywordbeta-Lactamases/analysis*-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameLee, Yang Soon-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.alternativeNameChong, Yun Sop-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorLee, Yang Soon-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.contributor.affiliatedAuthorChong, Yun Sop-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.citation.volume50-
dc.citation.number10-
dc.citation.startPage3227-
dc.citation.endPage3232-
dc.identifier.bibliographicCitationJOURNAL OF CLINICAL MICROBIOLOGY, Vol.50(10) : 3227-3232, 2012-
dc.identifier.rimsid33473-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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