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Evaluation of double-disk potentiation and disk potentiation tests using dipicolinic acid for detection of metallo-β-lactamase-producing pseudomonas spp. and Acinetobacter spp
DC Field | Value | Language |
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dc.contributor.author | 이경원 | - |
dc.contributor.author | 이양순 | - |
dc.contributor.author | 정석훈 | - |
dc.contributor.author | 정윤섭 | - |
dc.contributor.author | 용동은 | - |
dc.date.accessioned | 2014-12-19T17:03:23Z | - |
dc.date.available | 2014-12-19T17:03:23Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90694 | - |
dc.description.abstract | Accurate detection of metallo-β-lactamase (MBL)-producing Pseudomonas spp. and Acinetobacter spp. became very important with the increasing prevalence of carbapenem-nonsusceptible clinical isolates. The performance of phenotypic MBL detection methods may depend on the types of MBL and the characteristics of the isolates. A high false-positive rate is a problem with EDTA-based MBL detection methods. We evaluated the performance of double-disk potentiation tests (DDPTs) and disk potentiation tests (DPTs) with dipicolinic acid (DPA) using 44 isolates of Pseudomonas spp. and Acinetobacter spp. producing IMP-1-like, VIM-2-like, and SIM-1 type MBLs. Also, we characterized P. aeruginosa isolates with positive imipenem (IPM)-DPA DDPT, but negative meropenem (MEM)-DPA DDPT, and determined possibility of improving a DDPT by using MacConkey agar. Among five different DDPT methods, the IPM-DPA 250-μg method showed the highest sensitivity (97.7%) and specificity (100%). Among four DPT tests, the highest sensitivity (100%) was shown by the IPM-EDTA 1,900-μg disk method, but the specificity was very low (11.4%). Five of six P. aeruginosa isolates with false-negative DDPTs with MEM-DPA 250-μg disks carried bla(IMP-6,) and the high level resistance to MEM (MIC ≥ 512 μg/ml) was reduced by the presence of phenylalanine arginine β-naphtylamide. Improvement of DDPTs was observed when MacConkey agar was used instead of Mueller-Hinton agar. In conclusion, DPA is a better MBL inhibitor than EDTA for detection of Pseudomonas spp. and Acinetobacter spp. with IMP-1-like, VIM-2-like, and SIM-1-type MBLs. In DPA DDPTs, IPM disks perform better than MEM disks when the isolates are highly resistant to MEM due to the overexpression of efflux pumps. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF CLINICAL MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acinetobacter/enzymology* | - |
dc.subject.MESH | Acinetobacter/isolation & purification | - |
dc.subject.MESH | Acinetobacter Infections/microbiology | - |
dc.subject.MESH | Bacteriological Techniques/methods* | - |
dc.subject.MESH | Culture Media/chemistry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Picolinic Acids/metabolism | - |
dc.subject.MESH | Pseudomonas/enzymology* | - |
dc.subject.MESH | Pseudomonas/isolation & purification | - |
dc.subject.MESH | Pseudomonas Infections/microbiology | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | beta-Lactamases/analysis* | - |
dc.title | Evaluation of double-disk potentiation and disk potentiation tests using dipicolinic acid for detection of metallo-β-lactamase-producing pseudomonas spp. and Acinetobacter spp | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Yangsoon Lee | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.contributor.googleauthor | Yunsop Chong | - |
dc.identifier.doi | 22837321 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A02947 | - |
dc.contributor.localId | A03619 | - |
dc.contributor.localId | A03679 | - |
dc.contributor.localId | A02423 | - |
dc.relation.journalcode | J01325 | - |
dc.identifier.eissn | 1098-660X | - |
dc.identifier.pmid | 22837321 | - |
dc.subject.keyword | Acinetobacter/enzymology* | - |
dc.subject.keyword | Acinetobacter/isolation & purification | - |
dc.subject.keyword | Acinetobacter Infections/microbiology | - |
dc.subject.keyword | Bacteriological Techniques/methods* | - |
dc.subject.keyword | Culture Media/chemistry | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Picolinic Acids/metabolism | - |
dc.subject.keyword | Pseudomonas/enzymology* | - |
dc.subject.keyword | Pseudomonas/isolation & purification | - |
dc.subject.keyword | Pseudomonas Infections/microbiology | - |
dc.subject.keyword | Sensitivity and Specificity | - |
dc.subject.keyword | beta-Lactamases/analysis* | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.alternativeName | Lee, Yang Soon | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.alternativeName | Chong, Yun Sop | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lee, Yang Soon | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Chong, Yun Sop | - |
dc.contributor.affiliatedAuthor | Yong, Dong Eun | - |
dc.citation.volume | 50 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 3227 | - |
dc.citation.endPage | 3232 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL MICROBIOLOGY, Vol.50(10) : 3227-3232, 2012 | - |
dc.identifier.rimsid | 33473 | - |
dc.type.rims | ART | - |
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