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Dissemination of ceftazidime-resistant Acinetobacter baumannii clonal complex 92 in Korea

DC FieldValueLanguage
dc.contributor.author배일권-
dc.contributor.author이경원-
dc.contributor.author이양순-
dc.contributor.author정석훈-
dc.contributor.author김주원-
dc.date.accessioned2014-12-19T17:00:49Z-
dc.date.available2014-12-19T17:00:49Z-
dc.date.issued2012-
dc.identifier.issn1364-5072-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90615-
dc.description.abstractAIMS: This study was performed to describe the epidemiological traits of ceftazidime-resistant Acinetobacter baumannii clinical isolates from Korea. METHODS AND RESULTS: Antimicrobial susceptibilities were determined by disk diffusion assay. PCR experiments were performed to detect genes encoding extended-spectrum β-lactamases and metallo-β-lactamases. Detection of ISAba1 upstream of the bla(ADC) gene was also performed by PCR amplification. The genetic organization of the bla(PER-1) gene was investigated by PCR mapping and sequencing of the regions surrounding the gene. Multilocus sequence typing was performed using seven housekeeping genes. A. baumannii isolates of clonal complex (CC) 92 exhibited a higher resistance rate (286/289, 99%) against ceftazidime compared to A. baumannii isolates of non-CC92 (7/87, 8%). Amongst 286 ceftazidime-resistant isolates of CC92, 100 (35%) isolates carried the bla(PER-1) gene, while none of the 87 isolates of non-CC92 carried the gene. The bla(ADC) gene associated with an ISAba1 element was detected in 98% (281/286) of ceftazidime-resistant isolates of CC92 and in all seven ceftazidime-resistant isolates of non-CC92. The bla(PER-1) gene was located on a transposon, Tn1213 (ISPa12-bla(PER-1) -Δgst-ISPa13), in 95 isolates and on a complex class 1 integron (orf513-bla(PER-1) -putative ABC transporter gene) in five isolates. Southern blot experiments confirmed the chromosomal location of the bla(PER-1) gene. CONCLUSIONS: Acinetobacter baumannii CC92 which has acquired ceftazidime resistance by the production of PER-1 extended-spectrum β-lactamases and/or the overproduction of Acinetobacter-derived cephalosporinase is widely disseminated in Korea. SIGNIFICANCE AND IMPACT OF THE STUDY: This study shows the mechanisms of acquiring ceftazidime resistance in A. baumannii and the epidemiological traits of ceftazidime-resistant A. baumannii isolates from Korea.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF APPLIED MICROBIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcinetobacter Infections/microbiology*-
dc.subject.MESHAcinetobacter baumannii/drug effects*-
dc.subject.MESHAcinetobacter baumannii/genetics-
dc.subject.MESHAcinetobacter baumannii/isolation & purification-
dc.subject.MESHAcinetobacter baumannii/metabolism-
dc.subject.MESHAnti-Bacterial Agents/pharmacology*-
dc.subject.MESHCeftazidime/pharmacology*-
dc.subject.MESHCross Infection/microbiology*-
dc.subject.MESHDrug Resistance, Multiple, Bacterial*-
dc.subject.MESHHumans-
dc.subject.MESHIntegrons-
dc.subject.MESHKorea-
dc.subject.MESHMultilocus Sequence Typing-
dc.subject.MESHbeta-Lactam Resistance*-
dc.subject.MESHbeta-Lactamases/genetics-
dc.subject.MESHbeta-Lactamases/metabolism-
dc.titleDissemination of ceftazidime-resistant Acinetobacter baumannii clonal complex 92 in Korea-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorY. Lee-
dc.contributor.googleauthorI.K. Bae-
dc.contributor.googleauthorJ. Kim-
dc.contributor.googleauthorS.H. Jeong-
dc.contributor.googleauthorK. Lee-
dc.identifier.doi22404202-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01802-
dc.contributor.localIdA02649-
dc.contributor.localIdA02947-
dc.contributor.localIdA03619-
dc.contributor.localIdA00943-
dc.relation.journalcodeJ01238-
dc.identifier.eissn1365-2672-
dc.identifier.pmid22404202-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2012.05283.x/abstract-
dc.subject.keywordAcinetobacter Infections/microbiology*-
dc.subject.keywordAcinetobacter baumannii/drug effects*-
dc.subject.keywordAcinetobacter baumannii/genetics-
dc.subject.keywordAcinetobacter baumannii/isolation & purification-
dc.subject.keywordAcinetobacter baumannii/metabolism-
dc.subject.keywordAnti-Bacterial Agents/pharmacology*-
dc.subject.keywordCeftazidime/pharmacology*-
dc.subject.keywordCross Infection/microbiology*-
dc.subject.keywordDrug Resistance, Multiple, Bacterial*-
dc.subject.keywordHumans-
dc.subject.keywordIntegrons-
dc.subject.keywordKorea-
dc.subject.keywordMultilocus Sequence Typing-
dc.subject.keywordbeta-Lactam Resistance*-
dc.subject.keywordbeta-Lactamases/genetics-
dc.subject.keywordbeta-Lactamases/metabolism-
dc.contributor.alternativeNameBae, Il Kwon-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameLee, Yang Soon-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.alternativeNameKim, Ju Won-
dc.contributor.affiliatedAuthorBae, Il Kwon-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorLee, Yang Soon-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.contributor.affiliatedAuthorKim, Ju Won-
dc.citation.volume112-
dc.citation.number6-
dc.citation.startPage1207-
dc.citation.endPage1211-
dc.identifier.bibliographicCitationJOURNAL OF APPLIED MICROBIOLOGY, Vol.112(6) : 1207-1211, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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